中医药信息Issue(2)：16-18,3.
蛇床子素对海人酸致痫大鼠神经元 Caspase－3和Caspase－9蛋白表达的影响
Effect of Osthole on Expressions of Caspase-3 and Caspase-9 Proteins in Hippocampal Neuron in Kainite-induced Rats
摘要
Abstract
Objective: To investigate the effect of osthole on the hippocampal neuron and the expressions of Caspase-3 and Caspase-9 protein in the hippocampal neuron in kainite-induced rats.Methods:30 SD rats were divided into 3 groups,with 10 rats in each group, including a control group,a kainite group and an ost-hole group.Crystal violet staining was used to observe the morphological change of the hippocampal neuron. Immunohistochemistry staining was used to examine the expressions of Caspase-3 and Caspase-9 protein in the hippocampal neuron.Results: Crystal violet staining showed that the neurons of the hippocampal region exhibited irregular morphology and body shrinked, and reduced in numbers in the kainite group.However,the neurons in the osthole group showed clear cytoplasm dyed and nucleoli and similar number and morphology to the control group.Immunohistochemistry staining showed that the expressions of Caspase-3 and Caspase-9 in the hippocampal neuron in the kainite group increased significantly than those in the control group ( P<0.05 ) .The expressions of active Caspase-3 and active Caspase-9 of the hippocampal neuron in the Osthole group decreased obviously than that in the kainite group (P<0.05).Conclusion: Osthole has a protective effect on the hippocampal neurons in kainite -induced rats by inhibiting the activity of Caspase -3 and Caspase-9 protein.关键词
蛇床子素/海人酸/神经元/Caspase-3/Caspase-9Key words
Osthole/Kainite/Neuron/Caspase-3/Caspase-9分类
医药卫生引用本文复制引用
谢洪婷,沈阳,曾常茜..蛇床子素对海人酸致痫大鼠神经元 Caspase－3和Caspase－9蛋白表达的影响[J].中医药信息,2015,(2):16-18,3.基金项目
大连市科学技术基金计划项目 （）
