中医药信息Issue(2)：50-53,4.
纤维素酶提取金钗石斛多糖工艺研究
Cellulase Extraction Technology of Polysaccharide from Dendrobium Nobile
摘要
Abstract
ive:Objective:To optimize the parameters of the cellulose extraction of Polysaccharide from Dendrobi-um nobile .Mothods:Regard the extraction rate of polysaccharide as indexes,and use orthogonal experriments to determine the content of index in cellulose by UV Spectrophotometry.Then evaluate the merits of enzymatic parameters.Results:The optimized process conditions were solid-to-liquid ratio 1:50,pH 5.0,reflux time 170min,dosage of enzyme 4500U/g,enzymolysis temperature 55℃,and the Dendrobium nobile polysaccharide extraction rate was 7.71%,with 43.84% higher than conventional thermal reflow method.Conclusion:This method has advantages of simplicity,high extraction rate,which lays a foundation for the further study of Den-drobium nobile.It also has the potentially industrial application value in the extraction of effective ingredients of Chinese Medicine.关键词
金钗石斛/多糖/正交实验/纤维素酶Key words
Dendrobium nobile/Polysaccharides/Orthogonal experiment/Cellulase分类
医药卫生引用本文复制引用
张晓敏,孙志蓉,朱南南,程丽丽,杨春宁,王晨..纤维素酶提取金钗石斛多糖工艺研究[J].中医药信息,2015,(2):50-53,4.基金项目
“十一五”国家科技支撑计划项目 （）
