中医药信息Issue(4)：10-14,5.
左归丸对惊恐伤肾 MCAO大鼠 BDNF及其受体的影响
Effect of Zuogui Wan on BDNF and Its Receptor in MCAO Model Rats with“Panic Injured Kidney”
摘要
Abstract
Objective:To observe the influence of “panic injured kidney”on the cerebral ischemia injury and the expressions of BDNF and TrkB in MCAO model rats and the therapeutic effect of Bushen prescription of Zuogui Wan to explore the mechanism of action of Zuogui Wan and the neurobiological essence of“the kidney communicating to the brain” in the traditional Chinese theory.Methods:56 female Wistar rats were randomly divided into four groups and each group had 14 rats as following:a sham operation group,a model group,a“cat scaring mouse”model group and a treatment group.Middle cerebral artery occlusion( MCAO) was operated in rats except the rats in sham operation group,and the ischemia-reperfusion was used after 2 hours of MCAO.“Cat scaring mouse”model group and the treatment group were adopted to reproduce the animal models of“panic injured kidney”with 10 days prior to the operation of MCAO.The treatment group was given Zuogui Wan decoction one time a day for consecutively 10 days with 3 days before MCAO surgery.Immunohistochem-istry was used to assess the protein expressions of BDNF and P -TrkB/TrkB in MCAO rats.Results: Comparedwith the sham operation group, the protein expressions of BDNF and p -TrkB/TrkB increased in themodel group.Compared with the model group,the protein expressions of BDNF and p -TrkB/TrkB descendedin the“cat scaring mouse”model group.Compared with the“cat scaring mouse”model group, the protein expressionsof BDNF and p -TrkB/TrkB increased in the treatment group.Conclusion: The protein expressionsof BDNF and its receptor increase after cerebral ischemia,which may be a protective response of the body afterinjury.Kidney deficiency induced by panic can significantly attenuate the body's self -protection function aftercerebral injury and Zuogui Wan can prevent the impairment,which is likely to be achieved by upregulation ofthe protein expressions of BDNF and activation of TrkB.关键词
肾脑相关/脑缺血/恐伤肾/左归丸/BDNF/TrkBKey words
Kidney communicating to the brain/Cerebral ischemia/Panic injured kidney/Zuogui Wan/BDNF/TrkB分类
医药卫生引用本文复制引用
刘宏,贾彦,仲丽丽,孙世晓,张雨薇,郝迪,刘爽,叶阳,黄树明..左归丸对惊恐伤肾 MCAO大鼠 BDNF及其受体的影响[J].中医药信息,2015,(4):10-14,5.基金项目
国家自然科学基金青年基金项目（ No．81403288）；黑龙江中医药大学科研基金项目（ No．201315）；黑龙江省青年科学基金项目（ No． QC2015102）；黑龙江省博士后资助课题项目（No．LBH－Z14196）；黑龙江省青年科学基金项目 （）
