组织工程与重建外科杂志Issue(6)：324-328,5.DOI:10.3969/j.issn.1673-0364.2014.06.006
软骨形态发生蛋白1诱导的瘢痕成纤维细胞体内成软骨能力的实验研究
Chondrogenic Differentiation of Hypertrophic Scar -Derived Fibroblasts Initiated by Cartilage -derived Morphogenetic Protein 1 in Vivo
摘要
Abstract
Objective To explore the chondrogenesis potential of hypertrophic scar fibroblasts (HSFBs) induced by cartilage-derived morphogenetic protein 1 (CDMP1) in vivo. Methods Hypertrophic scar fibroblasts (HSFBs) were isolated from discarded hypertrophic scar. HSFBs combining with PGA/PLA scaffold as HSFBs- PGA/PLA complex were induced by CDMP1 (100 ng/mL) for 2 weeks. Then the complex were transplanted into nude mice as induced group (n=10). The complex that not induced were treated as negative control, named as un-induced group ( n=4), while the chondrocytes-PGA/PLA complex were treated as positive control, named as chondrocyte group (n=4). After 4 and 8 weeks, the complex in each group was harvested for wet weight test, proteoglycan (GAG) content test, HE staining, Safranine-O staining and immunochemistry staining. Results After 4 and 8 weeks in vivo, the wet weight and GAG content in induced group were both higher than in un-induced group (P<0.05). In induced group, after cultured for 4 weeks in vivo, chondrocyte-like lacuna structure was observed by HE staining, uniform distribution of GAG was observed by Safranine-O staining, and positive expression of collagen type Ⅱ was revealed by immunohistochemical staining. After 8 weeks of culturing in vivo, chondrocyte-like structure in induced group was more mature and more in line with the structure distribution of cartilage. Conclusion HSFBs combining with PGA/PLA had the potential to develop into polygonal chondrocyte-like tissue by the induction of CDMP 1.关键词
瘢痕成纤维细胞/软骨细胞/软骨形态发生蛋白1/诱导分化Key words
Hypertrophic scar fibroblasts/Chondrocytes/Cartilage-derived morphogenetic protein 1/Transdifferention分类
生物科学引用本文复制引用
沈聪聪,柴岗,曲淼,侯亦康,许祐荣,张艳..软骨形态发生蛋白1诱导的瘢痕成纤维细胞体内成软骨能力的实验研究[J].组织工程与重建外科杂志,2014,(6):324-328,5.基金项目
上海市科委自然科学基金（12ZR1416900）。 （）
