动物医学进展Issue(8)：11-16,17,7.
牛源肠出血性大肠埃希菌的分离鉴定及进化分析
Isolation,Identification and Phylogenetic Analysis of Enterohemorrhagic Escherichia coli from Cows
摘要
Abstract
To investigate the isolating rate of enterohemorrhagic Escherichia coli (EHEC)from cows and their phylogenetic relationships.EHEC were isolated and identified by polymerase chain reaction (PCR) and CT-SMAC separation.PCR was used to detect genes of stx1,stx2,eae,ehxA,saa,wzxO157.All i-solates were detected for the cytotoxicity in Vero cells.The isolation rate of EHEC was 7.1% (40/565). And those 40 EHEC belonged to 17 O serotypes,of which the O157 (4/40),O26 (4/40),O91 (7/40), O100 (4/40),O97 (6/40),O92 (2/40)were common serotypes.The isolation rates of stx1,stx2, ehxA,and saa were 82.5% (33/40),75% (30/40),90% (36/40),(27.5%11/40),respectively,which was higher than the other genes,e.g.eae (5%,2/40),wzxO157 (10%,4/40).All stains which carry stx1/stx2 showed cytotoxic activity in Vero cells.A total of 7 STs were identified in 40 isolates through MLST analysis,and ST297 was the common STs,was 57.5% (23/40).Phylogenetic analysis revealed a closer genetic relationship between our isolates and the EHEC strains which were epidemic in the world.It could be concluded that there exists molecular diversity among 40 EHEC,which showed genetic relation-ship with the popular EHEC worldwide.Except for EHEC O157,non-O157 EHEC strains could also threaten public health of humans,which should be monitored and detected frequently.关键词
肠出血性大肠埃希菌/分离与鉴定/多位点序列分型/序列型/进化分析Key words
Enterohemorrhagic Escherichia coli/isolation and identification/multilocus sequence typing/sequence type/phylogenetic analysis分类
农业科技引用本文复制引用
郭锐,谭臣,彭忠,华琳,曾东柱,吴斌..牛源肠出血性大肠埃希菌的分离鉴定及进化分析[J].动物医学进展,2015,(8):11-16,17,7.基金项目
国家自然科学基金重点项目 （）
