动物医学进展Issue(8)：28-34,7.
2010年－2014年猪流行性腹泻病毒S基因遗传进化分析
Phylogenetic Analysis of Porcine Epidemic Diarrhea Virus S Gene during 2010-2014
摘要
Abstract
Porcine epidemic diarrhea started widespread popularity in the immune pigs with the emergence of new strains since 2010,and quickly spread to many provinces in China.Its morbidity and mortality are still unable to get effective control.To clarify the genetic variation of the current popular strains,PEDV S gene sequences 319 strains from NCBI during 2010-2014 were compared by using DNA Star and MEGA6. The representative strains of six countries and regions were selected for sequence and phylogenetics analy-ses.Phylogenetic analysis showed that phylogenetic tree can be divided into two major branches,G1 and G2.Most of the pandemic strains are on the G1,and the genetic distance differs among prevailing strains, vaccine strains,classic strains.All the rest of the vaccine strains and classic strains are on G2,except for Chinju99 and JS/HZ/2012.The alignment results of nucleotide and amino acid sequences showed that the mutation sites are in pandemic strains,classical strains and vaccine strains,and focus on N end in S1. These results showed that a rapid variation and evolution of global PEDV strains occurred in recent years, so how to screen out the new vaccine candidate strains from the current epidemic strains will become the key for prevention of the porcine epidemic diarrhea.关键词
猪流行性腹泻病毒/S基因/遗传进化分析/变异Key words
Porcine epidemic diarrhea virus/S gene/phylogenetic analysis/variation分类
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刘艳成,芦婷,刘丹丹,李静,张文超,邓侨,张伟,杜雅楠..2010年－2014年猪流行性腹泻病毒S基因遗传进化分析[J].动物医学进展,2015,(8):28-34,7.基金项目
内蒙古自治区自然科学基金项目（980202）；内蒙古自治区2014年博士研究生科研创新重点项目 （）
