动物医学进展Issue(8)：35-39,5.
水貂阿留申病毒5个分离株的全基因测序及遗传变异分析
Genome Sequencing and Genetic Variation Analysis of 5 Isolated Strains of Aleutian Mink Disease Viruses
摘要
Abstract
The epidemiological surey showed that Aleutian mink disease is one of the three epideminc disea-ses to the mink farming industry.In spite of several attempts to provide an efficient protective protein based vaccine,experiment have failed so far.Based on published ADV sequences in GenBank,the 12 pairs of primers were designed for ADV in whole gene amplification and sequencing.The sequencing results were compared with the reference strains of ADV and the parvovirus subfamily representative species of each genus for nucleotide sequence and homology analysis.Whole genome amplification and sequencing re-vealed that the sequence lengths of the five strains were 4 537,4 539,4 562,4 572 and 4 569 bp,respec-tively.Nucleotide homology between the five isolated ADV strains and the European or American strains (ADV-G,ADV-Utahl,ADV-SL3)ranged from 92.8% to 97.6%.Based on the amino acid sequence de-duced from VP2 nucleotide of five isolates with DNASTAR software,we found that 61 amino acids were mutated.Three of the strains identified (ADV-G,ADV-DL124 and ADV-DL125)had a nine amino acid deletion at amino acid positions 26-35 and a one amino acid deletion at position 36 (glycine).The changes and deletions of amino acids did not affect the pathogenicity of the ADV.It was the foundation for the study of the Aleutian mink disease pathogenesis and Aleutian mink disease gene vaccine.关键词
水貂阿留申病毒/分离株/全基因/遗传变异Key words
Aleutian mink disease virus/isolate/genome/genetic variation分类
农业科技引用本文复制引用
刘东旭,李健明,时坤,曾范利,刘菲,杜锐..水貂阿留申病毒5个分离株的全基因测序及遗传变异分析[J].动物医学进展,2015,(8):35-39,5.基金项目
国家自然科学基金项目 （）
