动物医学进展Issue(8)：40-44,5.
小反刍兽疫病毒N蛋白B细胞表位预测、合成及间接ELISA方法的建立
Prediction of B Cell Epitopes,Synthesis And Development of Indirect ELISA for N Protein of Peste Des Petits Ruminants Virus
摘要
Abstract
Based on the amino acid sequence and the analysis results of secondary structures such as α-he-lix,β-sheet and turn regions,etc,the B cell epitopes for N protein of peste des petits ruminants virus were predicted by methods of Gamier-Robson and Chou-Fasman.Four artificial peptides were designed,synthe-sized and used as coating antigens,by which the indirect ELISA of serum antibody test for peste des petits ruminants virus was developed.The comparison test on goat clinical serum samples was carried out.Com-pared with the imported standard competitive ELISA,the sensitivity and specificity of the developed indi-rect ELISA were 96.0% and 96.25%,and the coincidence rate of the two methods was 96.15%.So the developed indirect ELISA method coating with synthetic peptides is suitable for clinical application.关键词
小反刍兽疫病毒/N蛋白/B细胞表位/酶联免疫吸附试验Key words
Peste des petits ruminants virus/N protein/B cell epitope/ELISA分类
农业科技引用本文复制引用
曾少灵,李希强,王红英,杨俊兴,花群义,阮周曦,唐金明,廖立珊,孙洁,林庆燕,吕建强,叶奕优,祝贺..小反刍兽疫病毒N蛋白B细胞表位预测、合成及间接ELISA方法的建立[J].动物医学进展,2015,(8):40-44,5.基金项目
“十二五”国家高技术研究发展计划项目（863计划）（2012AA101301）；“十二五”国家科技支撑计划项目（2013BAD12B03）；深圳检疫局科研项目（SZ2014203）；国家质检总局科研项目 （）
