动物医学进展Issue(8)：45-50,6.
猪流行性腹泻病毒流行毒株S基因的遗传变异和B细胞线性抗原表位分析
Genetic Variation and Linear B-cell Epitopes on Spike Gene of Porcine Epidemic Diarrhea Virus Fields Strains
摘要
Abstract
To explore the genetic variation characteristics of porcine epidemic diarrhea virus (PEDV)in re-cent years in China,a continuous RT-PCR was used to amplify the whole spike gene of 4 PEDV field strains.After sequencing and analyzing by some molecular software,there were two insertion sites at ami-no acid sites of 59-62(GENQ)and 140(N)and one deletion site at site of 163-164(NI)in the spike gene of fields stains with 4 161 bp(4 158)length.The phylogenetic tree showed that new field strains are disposed of a different group of G2 with other epidemic strains in recent years,while G1 group consists mostly of vaccines of CV777,DR13 and KPEDV-9 which share an identity of 92.4%-93.3% with field strains of spike gene at amino acid level.Linear B-cell epitope analysis showed that the threshold values at sites of 52-62,360-365 and 752-781 of field stains were high than that of vaccine strains,which indicated an intense relationship with pathogenicity,receptor-binding ability and infectivity.关键词
猪流行性腹泻病毒/S基因/遗传变异/突变/B细胞抗原表位Key words
Porcine epidemic diarrhea virus/S gene/genetic variation/mutation/B-cell epitope分类
农业科技引用本文复制引用
程小娜,王树双,李晓成,张志,樊雅婷,刘自立,吴发兴,刘爽,董雅琴,邵卫星,杨雨辉..猪流行性腹泻病毒流行毒株S基因的遗传变异和B细胞线性抗原表位分析[J].动物医学进展,2015,(8):45-50,6.基金项目
科技基础性工作专项项目（2012FY111000）；青岛市民生计划项目 （）
