动物医学进展Issue(8)：55-58,4.
EDTA 联合抗菌药对水霉菌生物膜的影响
Effects of EDTA Combining with Two Kinds of Antimicrobial Agents on Saprolegnia Biofilm
摘要
Abstract
The clean-up effect of EDTA,a metal-chelator,was investigated when used alone or combined with antimicrobial agents on Saprolegnia biofilm.The minimal inhibitory concentration (MIC)of EDTA to Saprolegnia spore was measured by microdilution broth method.Saprolegnia biofilms were treated by common and 2 times dose of anti-Saprolegnia drugs.Meanwhile,Saprolegnia biofilms were treated by 1 MIC,4 MIC and 16 MIC EDTA combining with common dose of methylene blue and Mei Ting,respective-ly.And then all results were measured by the CCK-8 (Cell Counting Kit-8).Furthermore,the biofilm treated by EDTA was stained with calcofluor white M2R,observed by fluorescence microscope,to investi-gate the change of the extracellular maxtrix.The results showed that,compared with the control group,it played no significant role when the biofilms were treated by common dose of methylene blue and Mei Ting respectively(P >0.05),the same as 2 times dose of methylene blue,but it changed when combining with EDTA.And the extracellular maxtrix of Saprolegnia biofilm reduced after being treated by EDTA.In conclusion,EDTA could destroy the structure of biofilm.The combination of EDTA with anti-Saproleg-nia drugs had significant activity to remove Saprolegnia biofilm.关键词
水霉菌/生物膜/乙二胺四乙酸/抗菌药/联合用药Key words
Saprolegnia/biofilm/ethylenediaminetetraacetic acid/antimicrobial agent/drug combination分类
农业科技引用本文复制引用
袁海兰,欧仁建,胡鲲,杨先乐..EDTA 联合抗菌药对水霉菌生物膜的影响[J].动物医学进展,2015,(8):55-58,4.基金项目
国家863计划项目（2011AA10A216）；上海市科技兴农重点攻关项目（沪农科攻字（2011）第4-8号）；国家水产种质资源平台运行服务项目；上海高校知识服务平台资助项目 （）
