法医学杂志Issue(4)：273-276,4.DOI:10.3969/j.issn.1004-5619.2015.04.005
GlobalFiler®PCR扩增试剂盒验证及其STR遗传多态性
Validation of GlobalFiler®PCR Amplification Kit and the STR Polymorphism
路志勇 1薛卢艳 1张庆霞 1赵怡 1刘金杰 1唐晖1
作者信息
- 1. 北京市公安司法鉴定中心，北京 100192
- 折叠
摘要
Abstract
Objective To test the technical param eters of GlobalFiler®PC R A m plification K it for its ap-plication to forensic application value and to investigate the genetic polym orphism s. Methods The valida-tion w as conducted in sensitivity, m ixed sam ples, species specificity, adaptability, survivability, consistency, peak height balance and stability. The am plification and detection of the genom ic D N A from 373 unre-lated individuals from B eijing H an nationality w ere extracted by autom ation w orkstation. Results Global-Filer®PC R A m plification K it w as adaptive to som e m ixed, degraded and inhibited sam ples. The pow er of sensitivity and adaptability and peak height balance show ed w ell. The distributions of genotype fre-quencies for 21 STR loci in the population w ere all in accordance w ith H ardy-W einberg equilibrium (P>0.05). The PIC value of the 21 STR loci w as am ong 0.536 to 0.940; the H value w as am ong 0.558 to 0.933; the D P value w as am ong 0.783 to 0.992; the PE value w as am ong 0.243 to 0.874. Conclusion GlobalFiler®PC R A m plification K it is suitable for crim inal cases and D N A database in forensic practice. A nd 21 STR loci in B eijing H an nationality have high polym orphism , w hich have ap-plication value in forensic practice and population genetics.关键词
法医遗传学/多态现象,遗传/短串联重复序列/GlobalFiler®PCR扩增试剂盒Key words
forensic genetics/polym orphism , genetic/short tandem repeat/G lobalFiler®PCR Amplifica-tion Kit分类
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路志勇,薛卢艳,张庆霞,赵怡,刘金杰,唐晖..GlobalFiler®PCR扩增试剂盒验证及其STR遗传多态性[J].法医学杂志,2015,(4):273-276,4.
