发育医学电子杂志Issue(2)：91-95,5.
组蛋白去甲基化酶 LSD1抑制剂对 MLL-ENL白血病细胞诱导分化的研究
LSD1 inhibition induces differentiation of MLL-ENL leukemia cells
摘要
Abstract
Objective To investigate the effect of histone demethylase LSD1 inhibition on MLL-ENL leukemia cells. Method An inducible MLL-ENL mouse leukemia cell line Csh3 was used as a model. The cells was first treated with LSD1 inhibitor TCP (Tranylcypromine) . The effect of MLL-ENL cells upon LSD1 inhibition were examined by cell proliferation assay, colony forming cell (CFC) assay and wright-giemsa staining. Furthermore, The global level of histone H3 lysine 4 dimethylation (H3K4me2) and the expression of specific oncogenes and differentiation related genes in MLL-ENL cells were determined using western blotting and RT-PCR respectively. Results LSD1 inhibition significantly reduced cell proliferation and colony growth, and induced cell differentiation in MLL-ENL leukemia cells. Conclusions LSD1 inhibition down-regulated oncogenes expression and up-regulated differentiation related genes to induce MLL-ENL leukemia cells differentiation. LSD1 inhibition may be a promising treatment for MLL-ENL leukemia.关键词
MLL-ENL白血病/组蛋白去甲基化酶LSD1/反式环苯丙胺/二甲基化/组蛋白H3赖氨酸4Key words
MLL-ENL leukemia/histone demethylase/LSD1/TCP/H3K4me2引用本文复制引用
杨雪静,和夫红,黄伊子,闫进,王前飞..组蛋白去甲基化酶 LSD1抑制剂对 MLL-ENL白血病细胞诱导分化的研究[J].发育医学电子杂志,2015,(2):91-95,5.基金项目
国家自然科学基金 （）
