广西植物Issue(4)：447-463,17.DOI:10.11931/guihaia.gxzw201506015
基于 ITS 和matK 序列的兰科沼兰族分子系统研究及二新种
Phylogenetic analysis of Malaxideae (Orchidaceae:Epidendroideae):two new species based on the combined nr DNA ITS and chloroplast mat K sequences
摘要
Abstract
Malaxideae is a larger cosmopolitan orchid tribe consisting of nearly 2 000 species and is distributed world-wide,excluding the polar and desert regions.It is abundant in the tropical regions,particularly in Australia,and the tropi-cal Asia,Southeast Asia,the tropical Americas and Africa.Molecular and morphological analyses have been performed to establish the phylogenetic relationships within this tribe.However,the subtribe and intergeneric relationships remain un-clear,and the genus definition of this tribe is considerably controversial.The maximum parsimony,maximum likelihood and Bayesian inference analyses were performed based on the combined sequences of the nuclear ITS and chloroplast mat K genes of 133 taxa (10 other allied species as outgroups),which represent nearly all of the major genera of the Mal-axideae.These results suggested that Malaxideae could be divided into three clades,the epiphytic Oberoniinae,the terres-trial Liparidinae,and a terrestrial Crepidium clade.The Oberoniinae and Liparidinae could be divided into six genera and five genera respectively,and Crepidium clade camposes of four genera.The subtribe Ypsilorchidinae should be attributed to Oberoniinae.Disticholiparis should be removed because it shared the same type with Stichorkis .The phylogenetic re-lationship of Crepidium and Dienia is monophyletic,although they have different lip structures.In addition,two new species found in Southwest China and northern Vietnam,Platystyliparis malipoensis G.D.Tang ,X.Y.Zhuang & Z.J. Liu and Cestichis pingtaoi G.D.Tang ,X.Y.Zhuang & Z.J.Liu,were described based on the molecular analysis and morphological observation.关键词
沼兰族/核基因 ITS/mat K/系统学/并系类群Key words
Malaxideae/nrDNA ITS/mat K/phylogeny/Paraphyletic分类
农业科技引用本文复制引用
唐光大,张国强,洪文君,刘仲健,庄雪影..基于 ITS 和matK 序列的兰科沼兰族分子系统研究及二新种[J].广西植物,2015,(4):447-463,17.基金项目
国家林业发展计划项目(2011-100) （2011-100）
广东省自然科学基金(2014A030310465) （2014A030310465）
深圳市科技计划项目(JC201005310689A) （JC201005310689A）
