郑州大学学报（医学版）Issue(4)：481-484,4.DOI:10.13705/j.issn.1671-6825.2015.04.008
乳癌患者转移淋巴结中T细胞受体β链互补决定区3谱型分析∗
Analysis of complementarity determining region 3 repertoire of T cell re-ceptor β chain in metastatic lymph node from patients with breast cancer
摘要
Abstract
Aim: To analyze the specific repertoire skewing and amino acid sequences of the complementarity determi-ning region 3(CDR3) of T cell receptor(TCR) β chain in metastatic lymph node from patients with breast cancer. Methods:A total of 24 TCR β chain variable region(BV) subfamilies were amplified by RT-PCR, and the CDR3 lengths were analyzed with GeneScan method in lymph nodes from 2 patients with reactive hyperplasia and metastatic lymph nodes from 5 patients with breast cancer. Sequence analysis of the CDR3 region in monoclonal or oligoclonal expanded T cells was performed. Re-sults: There were only multiclonal expanded T cells in lymph nodes from patients with reactive hyperplasia, while there were monoclonal, oligoclonal, and multiclonal expanded T cells in metastatic lymph nodes from patients with breast cancer. TCR BV presented specific repertoire skewing in metastatic lymph node from patients with breast cancer, among which only 2-5 TCR BV subfamilies were identified. The monoclonal or oligoclonal expanded T cells had different CDR3 amino acid se-quences. Conclusion: There is specific repertoire skewing of TCR BV subfamilies in metastatic lymph nodes from patients with breast cancer. The amino acid sequences of CDR3 in different T cell clone proliferation are mostly different.关键词
乳腺肿瘤/T细胞受体/互补决定区3Key words
breast neoplasm/T cell receptor/complementarity determining region 3分类
医药卫生引用本文复制引用
张建波,吕晓东,宋巍,孙淼淼,于庆凯,冯稳,刘明阁,胡骏,房百俊..乳癌患者转移淋巴结中T细胞受体β链互补决定区3谱型分析∗[J].郑州大学学报（医学版）,2015,(4):481-484,4.基金项目
国家自然科学基金资助项目81370661 （）
河南省基础与前沿技术研究计划基金资助项目102300410038 （）
