中医学报Issue(9)：1329-1331,3.DOI:10.16368/j.issn.1674-8999.2015.09.459
基于清除 DPPH·法研究清热解毒中药的体外抗氧化活性
Comparative Research the Antioxidant Activity of Ten Samples of Heating-clearing and Detoxicating Drugs in Vitro by DPPH·
摘要
Abstract
Objective:To give some new advises and insights to the research of new traditional Chinese medicine,we measured the antiox-idant activity in vitro of ten samples of heating-clearing and detoxicating drugs. Methods:Use microplate method to determine the antiox-idant activity by cleaning DPPH· and calculate the half inhibition concentration(IC50 ). If the value of IC50 was smaller,it indicated the antioxidant activity was stronger. Results:Different traditional Chinese medicine has different antioxidant activity in vitro,the dose-efficiency curve shows that the potency and efficacy of forsythia was strongest and the IC50 of forsythia was 0. 3127 mg·mL - 1 that of smallest. Conclusion:The antioxidant activity of forsythia was strongest. It provides a new way to research the mechanism of action of for-sythia for treating diabetes and application prospect.关键词
清热解毒药/连翘/DPPH·法/抗氧化活性Key words
heating-clearing and detoxicating drugs/forsythia/DPPH· method/antioxidant activity分类
医药卫生引用本文复制引用
吴宿慧,李竹,张颜语,李寒冰..基于清除 DPPH·法研究清热解毒中药的体外抗氧化活性[J].中医学报,2015,(9):1329-1331,3.基金项目
国家自然科学基金面上项目（81473435）；河南省教育厅科学技术重点资助项目（14A310023）；河南中医学院大学生创新学习项目｛CXXM［2014］14｝；河南省科技厅科技攻关重点项目（144300510015）；河南中医学院博士科研基金项目 （）
