黑龙江农业科学Issue(7)：56-61,6.DOI:10.11942/j.issn1002-2767.2015.07.0056
土壤拮抗细菌的分离与抗植物病原真菌活性初步研究
Preliminary Study on Isolating Antagonistic Bacteria f rom Soil and Antagonistic Activity Against Plant Pathogens
摘要
Abstract
In order to screen antagonistic bacteria in soil for plant pathogens bio‐control .55 antagonistic bacteria strains were isolated from soil samples in different ecological regions by the soil sprinkling method baited with 4 soil borne diseases .Part of antagonistic bacteria strains were evaluated for the antagonistic activity against plant pathogens .The result showed that more than 60% mycelial inhibition against Fusarium solani ,Fusarium graminearum ,Fusarium oxysporum were 1 ,12 and 8 stains ,respectively .Fermentation filtrate of 9 antagonis‐tic bacteria strains showed higher inhibition effect for Sclerotinia sclerotiorum ,Pyricularia grisea ,Setospha‐eriaturcica,Bipolarissorokiniana,FusariummoniliformeandAlternariasolani,thefermentationproductsof YS7 strain showed strong antifungal activity to Setosphaeria turcica ,Bipolaris sorokiniana ,Fusarium monili‐forme ,the diameter of inhibition zone reached a maximum of 56 .2 mm against Setosphaeria turcica .Metabo‐lites of 27 antagonistic bacteria strains showed inhibition activity to more than 4 antagonistic objects ,it showed that the fermentation products of antagonistic bacteria strains had broad‐spectrum antifungal activity .关键词
拮抗细菌/土壤/植物病原真菌/抑菌活性/代谢产物Key words
antagonistic bacteria/soil/plant pathogens/antagonistic effect/fermentation products分类
农业科技引用本文复制引用
刘春来,李新民,王爽,夏吉星,杨帆,刘宇,苏宝华..土壤拮抗细菌的分离与抗植物病原真菌活性初步研究[J].黑龙江农业科学,2015,(7):56-61,6.基金项目
国家高技术研究发展计划（863计划）资助项目 （）
