Abstract
Objective To investigate the phylogenetic analysis among a group of drug‐resistant Acinetobacter baumannii (A .Baumannii) .Methods From January 2012 to December 2013 ,20 strains of drug‐resistant A .bauman‐nii were collected from a hospital in China .Then ,resistance genes were analyzed by PCR ,including 3 kinds of house‐keeping genes (carO ,gyrA ,parC)and 54 kinds of resistance genes acquired by horizontal transfer to beta‐lactams , aminoglycosides ,quinolones ,as well as 12 kinds of genetic markers of conjugal plasmids ,transposons ,insertion se‐quences ,and integrons .In addition ,sample cluster analysis were performed .Results In 20 strains of drug‐resistant A .baumannii ,3 kinds of housekeeping genes (carO ,gyrA ,parC ) ,6 kinds of acquired beta‐lactam‐resistance genes (TEM‐1 ,PER ,ADC‐30 ,ADC‐58 ,ADC‐59 ,OXA‐23) ,5 kinds of acquired aminoglycoside‐resistance genes (aac(3)‐Ⅰ ,aac(6′)‐Ⅰb ,ant(3")‐Ⅰ ,aph(3′)‐Ⅰ ,armA) ,2 kinds of drug efflux genes (adeB ,qacE△1) ,7 kinds of genetic markers of mobile genetic elements (intⅠ1 ,tnpU ,tnp513 ,IS26 ,IS903 ,ISaba1 ,ISaba9) were positive .And sample cluster analysis showed that these strains were divided into 2 clusters :cluster A (multidrug‐resistant A .baumannii) and cluster B(pandrug‐resistant A .baumannii) .Conclusion The relationships among Strain1 ,2 ,13 ,17 ,20 and Strain 10 ,14 ,16 in cluster A were close ,and Strain 4‐6‐8‐9‐15‐19 were the same clone .So it′s important to monitor and con‐trol noscomial infection intime depending on phylogenetic analysis among strains .关键词
鲍曼不动杆菌/β-内酰胺类/氨基糖苷类/喹诺酮类/菌株亲缘性/样本聚类分析/水平转移基因Key words
acinetobacter baumannii/beta-lactams/aminoglycosides/quinolones/relatedness/sam-ple cluster analysis/horizontal transfer gene
