摘要
Abstract
Objective To investigate the influence of smoking on disease progression and recurrence in elderly patients with chronic periodontitis .Methods 50 cases of chronic periodontitis patient in our hospital from January 2012 to January 2014 were selected ,among them 25 cases of smoking were taken as the observation group and 25 ca‐ses of non‐smoking as the control group .5mL of forearm blood was collected in 50 patients and centrifuged for ex‐tracting serum .The serum 200 ,400 ,800 μL in smoking individual and non‐smoking individuals were added to porphy‐romonas gingivalis(Pg) infecting the KB cell model ,after 12 h action ,the brain heart infusion agar medium was used for conducting 5 - 7 d bacterial culturing and the bacterial colonies were counted .The human enzyme‐linked immu‐nosorbent assay(ELISA) kit was used to detect the concentration differences of matrix metalloproteinases(MMP)‐1 , MMP‐9 and tissue inhibitor of metalloproteinase 1 (TIMP‐1) in the supernatant of two groups .The influence of adding different volumes of serum on the Pg internalizing KB cells and the differences of KB cells secreting MMP‐1 , MMP‐9 and TIMP‐1 were analyzed .In adding the same volume of serum ,Pg internalizing KB cells and difference of KB cells secreting MM P‐1 ,MMP‐9 and TIMP‐1 were compared between the two groups .Results After adding dif‐ferent volumes of serum ,the colonies of Pg internalizing KB cells in control group individual serum were significantly less than those in the smoking group ,the difference was statistically significant(P< 0 .05) .In adding different vol‐umes of serum in the two groups individual serum ,the difference of colonies number of Pg internalizing KB cells were statistically significant(P< 0 .05) .With increasing doses of serum in the smoking group ,KB cells expressed MMP‐1 , MMP‐9 and TIMP‐1 concentrations were increased ,the MM P‐1 ,MMP‐9 and TIMP‐1 expression differences caused by different volumes of serum were statistically significance(P< 0 .05) .Comparing adding 800 μL of smoking group serum with 800 μL of non‐smoking group serum ,KB cells secreting MMP‐1 ,MMP‐9 and TIMP‐1 concentrations were significantly increased(P< 0 .05) .Conclusion Smoking individual serum can contribute to Pg internalizing KB cells to produce MM P‐1 ,MMP‐9 and TIMP‐1 ,thus promote the progression and recurrence of chronic periodontitis .关键词
吸烟/慢性牙周炎/基质金属蛋白酶类Key words
smoking/chronic periodontitis/matrix metalloproteinases
