临床神经外科杂志Issue(4)：274-278,5.DOI:10.3969/j.issn.1672-7770.2015.04.009
miR-150*修饰骨髓间充质干细胞来源的 exosome对胶质瘤细胞的影响
miR150*-Loaded exosomes from marrow stromal cells could inhibit glioma cells growth
摘要
Abstract
Objective MiRNA-based therapeutics hold great promise for tumor suppression, this study was to investigate the effect of miR-150*-loaded exosomes on regulation of glioma cells proliferation and cell cycle.Methods Quantitative real-time PCR on 15 glioblastoma tissues samples and normal controls were used to confirm the miR-150* expression level.Western blotting analysis and electron microscopy were employed to test exosomal biomarkers and their morphology. Transfection assay were used to collect miR-150*-loaded exosomes from bone marrow mesenchymal stem cells (BMSCs)culture medium.CCK-8 and cell cycle assays were used to analyze miR-150*-loaded exosomes effects on glioma cells.Results Level of miR-150* expression was much lower in glioblastoma than in normal tissues.Transfection assay successfully acquired miR150*-loaded exosomes which derived from bone marrow mesenchymal stem cells (BMSCs).Furthermore,miR-150*-loaded exosomes could largely inhibited glioma cells proliferation and suppress cell cycle progression.Cell counting kit 8 (CCK-8)assays also demonstrated miR-150* delivered in exosomes was much less toxic.Conclusions This study demonstrated miR-150* is down-regulated in glioblastoma.miR-150*-loaded exosomes could suppress glioma cells and exosomes may be a potentially efficient therapeutic delivery system.关键词
miR-150*/胶质瘤/exosome/增殖Key words
miR-150*/glioma/exosome/proliferation分类
医药卫生引用本文复制引用
廖克曼,季卫阳,鲁晓杰..miR-150*修饰骨髓间充质干细胞来源的 exosome对胶质瘤细胞的影响[J].临床神经外科杂志,2015,(4):274-278,5.基金项目
无锡市医管中心重大项目基金资助项目 （）
