农业环境科学学报Issue(7)：1384-1391,8.DOI:10.11654/jaes.2015.07.022
菌渣纤维素降解菌的筛选与鉴定
Screening and Identification of Cellulose-Degrading Bacteria from Spent Substrate of Edible Mushroom
摘要
Abstract
The cellulose in spent substrate of edible mushroom is often a limiting factor of the spent substrate decomposition. This study was performed to isolate highly effective cellulose-degrading bacteria from spent substrate in order to improve the fermentation of spent substrate of edible mushroom. Samples were collected from spent substrates of Pleurotus eryngii at different stacking places. Four strains(FB7, CB1, BC11 and BC12)with high cellulose-degrading efficiencies were obtained based on the results of Congo red cellulose hydrolytic hole mea-surement, filter paper degradation test and cellulose activity assays(filter paper enzyme FPA, endoglucanase CMCase, exoglucanase C1 and glucosidaseβ-Gase), using CMC-Na as sole carbon source. 16S rDNA sequencing showed that the trains FB7 and CB1 were Bacillus sub-tilis,thestrainBC11wasStreptomycesalbus,andthestrainBC12wasComamonas jiangduensis.ThestrainFB7withhighfilterpaperde-grading ability had high cellulose activities and degraded filter paper into paste within 10 days. The activities of four enzymes(FPA, CM-Case, C1 andβ-Gase)produced by the stain FB7 cultured for 4 days on a shaking incubator were 22.81 U·g-1, 314.50 U·g-1, 2.78 U·g-1 and 188.09 U·g-1, respectively. The FPA, CMCase, C1, andβ-Gase activities of bacterial combination(CB1+BC11+FB7)were 31.56 U·g-1, 133.61 U·g-1, 2.31 U·g-1, and 217.21 U·g-1, increased by 38.4%, 11.2%, 178%, and 70.3%, respectively, compared with the strain FB7 alone. Inoculation of bacterial combination(CB1+BC11+FB7)to spent substrate compost increased the temperature of the compost quickly, with higher and lesser fluctuations than in the control.关键词
菌渣/纤维素降解菌/筛选/16S rDNA序列/酶活力/复合菌剂/堆肥Key words
spent substrate/cellulose degrading microorganisms/screening/16S rDNA sequence/enzymatic activity/complex bacteria/compost分类
资源环境引用本文复制引用
刘晓梅,邹亚杰,胡清秀,杨小红,沈德龙..菌渣纤维素降解菌的筛选与鉴定[J].农业环境科学学报,2015,(7):1384-1391,8.基金项目
“十二五”国家科技支撑计划课题“基于农牧废弃物-食用菌开发体系的农业生境过程控制”（2012BAD14B15-7）；中国农业科学院科技创新工程 （）
