山东农业科学Issue(8)：1-5,5.DOI:10.14083/j.issn.1001-4942.2015.08.001
滨麦草与百萨偃麦草 PHR1基因的克隆及序列分析
Cloning and Sequence Analysis of PHR1 Gene from Leymus mollis and Thinopyrum bessarabicum
摘要
Abstract
To fully explore the plant potential for high -efficiency phosphorus utilization and enhance the plant absorption and use efficiency of insoluble phosphorus in soil will play important roles in breeding new va-rieties,reducing fertilizer input and protecting agro -ecological environment.PHR1 is an important transcrip-tion factor of multiple Pi starvation responses in Pi signaling pathways.By homologous cloning,two PHR1 genes were cloned from Elymus mollis and Thinopyrum bessarabicum,which were named as LmPHR1 and Tb-PHR1.Their ORFs were both 1 356 bp,and both of them encoded 451 amino acids;their cDNA sequences shared 98.63% identity with the cDNA sequences of Xiaoyan 54 PHR1 -A1,PHR1 -B1,PHR1 -D1.Their encoded proteins both had a MYB DNA binding domain and a predicted coiled -coil domain.Using the Clust-alX for phylogenetic analysis of the two PHR1 genes with the reported PHR1 genes in rice and wheat,ect.The results showed that PHR1 had closer evolutionary relationship with the PHR1s from Brachypodium distachyon, Oryza sativa,Zea mays and Arabidopsis thaliana;while the LmPHR1 had more faraway evolutionary relation-ship with other plants’PHR1 genes.关键词
滨麦草/百萨偃麦草/PHR1基因/同源克隆Key words
Leymus mollis/Thinopyrum bessarabicum/PHR1 gene/Homologous clone分类
农业科技引用本文复制引用
张雪莹,李太强,王洪刚,李兴锋..滨麦草与百萨偃麦草 PHR1基因的克隆及序列分析[J].山东农业科学,2015,(8):1-5,5.基金项目
国家转基因生物新品种培育重大专项（2011ZX08002－005）；山东省良种工程农业资源创新利用课题 （）
