上海农业学报Issue(4)：34-39,6.DOI:10.15955/j.issn1000-3924.2015.04.07
基于多重 PCR 的主要食源性致病菌的快速检测
Rapid detection of foodborne pathogenic bacteria based on multiple PCR
摘要
Abstract
According to the specific sequence of 4 common foodborne pathogenic bacteria,Listeria monocy-togenes,Salmonella enterica,Staphylococcus aureus and Escherichia coli O157:H7,4 pairs of specific primers were designed and a multiple PCr method for rapid detecting the above bacteria was established.The additive amount of template,primers,rTaq,MgSO4 ,and the annealing temperature of the reaction system were optimized.The re-sults showed that the specificity of 4 pairs of primers was better.The sensitivity of 4 species of pathogenic bacteria was detected by single PCr,and the detection limit was 103 copies/mL.The sensitivity of multiple PCr for simul-taneous detection of 4 species of pathogenic bacteria was 103 copies/mL,and the sensitivity of 4 species of patho-genic bacteria in artificial contaminated pork was detected as 103 cfu/mL.The detection method did not produce cross reactions with other bacteria.Compared with the traditional method,the detection time was greatly short-ened,and the accuracy of detection was improved.关键词
多重 PCr/食源性致病菌/检测/食品安全Key words
Multiple PCr/Foodborne pathogenic bacteria/Detection/Food safety引用本文复制引用
胡瑞丽,任方方,吴洋洋,许燕,赵凯,索玉娟,邵毅,周昌艳..基于多重 PCR 的主要食源性致病菌的快速检测[J].上海农业学报,2015,(4):34-39,6.基金项目
上海市闵行区产学研项目（2013MH099）；上海市科技新农重点攻关项目［沪农科攻字（2013）第3-2号］ （）
