生物技术通报Issue(8)：166-173,8.DOI:10.13560/j.cnki.biotech.bull.1985.2015.08.024
绿针假单胞菌HT66中luxR_19对吩嗪-1-甲酰胺合成的影响
The Effects ofluxR_19 on the Synthesis of Phenzaine-1-carboxamide in Pseudomonas chlororaphis HT66
摘要
Abstract
In order to study the potential effects of regulator geneluxR_19 on the synthesis of antibacterial substance phenazine-1-carboxamide(PCN)and physiological-biochemical characteristics inPseudomonas chlororaphis HT66, strains of geneluxR_19-knockout,-complementation,-overexpression and -point-mutation were constructed, and then the growth curve, fermentation production of PCN, swimming and swarming activities were tested at the target strains. The results showed that the knockout ofluxR_19 lowered the production of PCN and the over-expression of geneluxR_19 increased the double production of PCN compared to the wild type;luxR_19 gene affected the expression of several genes relating to the regulation or synthesis of phenazines. Besides, the knockout ofluxR_19 had no effects on the growth and swimming activity, however, promoted the swarming activity of HT66. The production of PCN in point-mutation strain ofluxR_19(G85A)was similar to that in wild type. The above results indicated thatluxR_19 was the positive regulator of PCN production, and could affect the swarming activity of HT66. Besides, the point-mutation at 254th site inluxR_19 did not affect the production of PCN.关键词
绿针假单胞菌/HT66/吩嗪-1-甲酰胺/LuxR家族转录调控因子Key words
Pseudomonas chlororaphis/HT66/phenazine-1-carboxamide/transcriptional regulator of LuxR family引用本文复制引用
陈亚汶,彭华松,王威,张雪洪..绿针假单胞菌HT66中luxR_19对吩嗪-1-甲酰胺合成的影响[J].生物技术通报,2015,(8):166-173,8.基金项目
国家自然科学基金项目（31270084），国家“863”计划项目 （）
