生物技术通报Issue(8)：174-179,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.08.025
高产二羟基丙酮重组菌株的构建
Construction of a Recombinant Strain Producing High-yield Dihydroxyacetone
摘要
Abstract
This work aims to construct a recombinant strain containing genesldAB over-expressing membrane-bound glycerol dehydrogenase for raising the yield of dihydroxyacetone(DHA). Firstly, using genomic DNA ofGluconobacter oxydansATCC621H as a template,sldAB amplified by PCR was ligated to the plasmid pBBR1MCS-2 and the expression vector pBBR1MCS-2-sldAB was constructed;subsequently the plasmid pBBR1MCS-2-sldAB was transformed into theG. oxydans ATCC621H by electrotransfer, and the recombinant strain GOX205 was obtained. Results showed that the recombinant strain was constructed successfully, and the activity of glycerol dehydrogenase increased by 26% compared with the original strain. When initial concentration 100 g/L of glycerol as carbon source, the growth of GOX205 was significantly improved compared with the original strain, the final DHA concentration was 94.1 g/L and increased by 19.7%, and the residue of glycerol decreased 15.1 g/L. Therefore, this study provides a basis for further improving the biotransformation process of DHA production.关键词
1,3-二羟基丙酮/重组菌株/甘油/过量表达Key words
1, 3-dihydroxyacetone/recombinant strain/glycerol/over-expression引用本文复制引用
许晓菁,赵琼,王祥河..高产二羟基丙酮重组菌株的构建[J].生物技术通报,2015,(8):174-179,6.基金项目
天津市应用基础与前沿技术研究计划（天津市自然科学基金）重点项目 （）
