生物技术通报Issue(8)：180-185,6.DOI:10.13560/j.cnki.biotech.bull.1985.2015.08.026
重组精氨酸脱亚胺酶制备L-瓜氨酸的工艺条件优化
Optimization of Preparing L-citrulline by Recombinant Arginine Deiminase
马越 1宿玲恰 2吴丹 1吴敬2
作者信息
- 1. 江南大学 食品科学与技术国家重点实验室，无锡 214122
- 2. 江南大学生物工程学院 工业生物技术教育部重点实验室，无锡 214122
- 折叠
摘要
Abstract
ThearcAgene encoding ADI from Pseudomonas putida ACCC 10185 was cloned into the expression vector pET-24a (+). The vector was then transformed intoEscherichia coli BL21(DE3)for intracellular production of ADI. The crude enzyme was obtained by ultrasonic treatment, and activity in the fermentation broth of recombinantE. coli BL21(DE3)was 26 U/mL. Furthermore, the condition for enzymatic conversion of L-arginine monohydrochloride to L-citrulline by the recombinant ADI was optimized. At 650 g/L of L-arginine monohydrochloride, pH6.0, 37℃, 100-200 r/min, and 24 U ADI per gram substrate incubated for 7 hours, 100% of the L-arginine monohydrochloride was transformed into L-citrulline, which was the highest level of preparing L-citrulline by enzyme method in home and abroad presently.关键词
精氨酸脱亚胺酶/L-瓜氨酸/酶转化/重组表达Key words
arginine deiminase/L-citrulline/enzymatic conversion/recombinant expression引用本文复制引用
马越,宿玲恰,吴丹,吴敬..重组精氨酸脱亚胺酶制备L-瓜氨酸的工艺条件优化[J].生物技术通报,2015,(8):180-185,6.
