外科理论与实践Issue(4)：327-332,6.DOI:10.16139/j.1007-9610.2015.04.012
铜绿假单胞菌对胰腺癌细胞的抑癌效应及分子机制研究
Study on anti-cancer effects of pseudomonas aeruginosa for pancreatic cancer cells and molecular mechanism
摘要
Abstract
Objective To investigate the anti-cancer effects of pseudomonas aeruginosa for pancreatic cancer cells and the mechanism. Methods CCK8 method was used to detect cell proliferation rate after pancreatic cancer cells were treated with pseudomonas aeruginosa. The cell ultrastructure were observed with transmission electron microscope. The apoptosis and cycle of cell was detected with Annexin Ⅴ/PI flow cytometry after treatment of pancreatic cancer by pseu-domonas aeruginosa with different concentrations. The expression of several proteins associated with epidermal growth fac-tor receptor (EGFR) signaling pathway was detected using Western blot. Results It was observed that pseudomonas aerugi-nosa significantly inhibited the proliferation of pancreatic cancer cells and the changes of apoptotic morphology were found. After treating with pseudomonas aeruginosa, the apoptosis of tumor cells increased significantly and the tumor cell cycle arrest was induced and the expression of EGFR signaling pathway-associated phosphorylated proteins of tumor cells decreased significantly. Conclusions Pseudomonas aeruginosa induces apoptosis of pancreatic cancer cells combined with cell cycle arrest and inhibits tumor growth. Inhibition of EGFR pathway might be an important mechanism in inducing apoptosis of pancreatic cancer cells.关键词
胰腺癌/铜绿假单胞菌/周期阻滞/凋亡/EGFR通路Key words
Pancreatic cancer/Pseudomonas aeruginosa/Cell cycle arrest/Apoptosis/EGFR pathway分类
医药卫生引用本文复制引用
汪炳瑞,程兮,杨卫平,景晓乾,马丁,刘心玉,沈柏用,彭承宏,邱伟华..铜绿假单胞菌对胰腺癌细胞的抑癌效应及分子机制研究[J].外科理论与实践,2015,(4):327-332,6.基金项目
国家自然科学基金（81172326）；上海慈善癌症研究基金 （）
