中国组织工程研究Issue(28)：4439-4444,6.DOI:10.3969/j.issn.2095-4344.2015.28.003
龙鳖胶囊对骨髓间充质干细胞增殖活性的影响
Effect of Longbie Capsule on proliferation of bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:Longbie Capsule has satisfactory outcomes in the treatment of osteoarthritis, but its mechanism is stil unclear. <br> OBJECTIVE:To study the effect of Longbie Capsule on proliferation of bone marrow mesenchymal stem cel s. METHODS:The bone marrow mesenchymal stem cel s from SD rats were separated and expanded by whole adherence culture, then subcultured and confirmed by morphological observation and flow cytometry. Passage 4 cel s were cultured in complete media containing 5 g/L, 1 g/L, 250 mg/L, 50 mg/L, 10 mg/L Longbie Capsule, respectively, for 24 hours. Then, MTT assay was used to detect cel viability. <br> RESULTS AND CONCLUSION:The primary cel s were adherent cel s characterized by irregular shape, passage 2 cel s were typical y fibrous-shaped, passage 3 cel s grew in long fibrous and swirl-type shape. Passage 4 cel s were strongly positive for CD29 and CD90, positive for CD44, and negative for CD34 and CD45. 5 g/L and 1 g/L Longbie Capsule promoted the proliferation of bone marrow mesenchymal stem cel s. These findings indicate that Longbie Capsule may promote the proliferation and differentiation of bone marrow mesenchymal stem cel s, thereby playing a therapeutic effect on osteoarthritis.关键词
干细胞/骨髓干细胞/龙鳖胶囊/骨髓间充质干细胞/骨关节炎/补肾活血/MTT/细胞增殖/国家自然科学基金分类
医药卫生引用本文复制引用
潘建科,郭柏铭,刘军,张娴,谢辉,郭达..龙鳖胶囊对骨髓间充质干细胞增殖活性的影响[J].中国组织工程研究,2015,(28):4439-4444,6.基金项目
国家自然科学基金项目(81473698，81273781)；教育部高等学校博士点科研基金项目(20124425110004)；广东省科技计划项目(2011B031700027)；广东省财政厅项目([2014]157号；广东省中医院中医药科学技术研究专项资助(YK2013B2N19)@@@@Funding:the National Natural Science Foundation of China, No.81473698,81273781 （81473698，81273781）
the Specialized Research Fund for the Doctoral Program of Higher Education, Ministry of Education, No.20124425110004 （）
the Science and Technology Plan of Guangdong Province, No.2011B031700027 （）
the Project of Guangdong Finance Department, No.[2014]157 （）
the Specialized Fund for Chinese Medicine Research in Guangdong Provincial Hospital of Chinese Medicine, No. YK2013B2N19 （）
