中国组织工程研究Issue(28)：4450-4454,5.DOI:10.3969/j.issn.2095-4344.2015.28.005
姜黄素对骨髓间充质干细胞诱导肾移植免疫耐受的影响
Effects of curcumin on mesenchymalstem cells-induced immune tolerance to kidney transplantation
摘要
Abstract
BACKGROUND:Previous studies have shown that continuous administration of 1×107 of bone marrow mesenchymal stem cel s can induce immune tolerance in rats undergoing kidney transplantation, but it is not yet found clinical y that curcumin exerts an on immunomodulatory effect on kidney transplantation. <br> OBJECTIVE:To investigate the effects of different concentrations of curcumin on bone marrow mesenchymal stem cel s-induced immune tolerance in rats after kidney transplantation. <br> METHODS:Rat model of kidney transplantation was made, and rat models were randomly divided into four groups:transplantation group with no treatment;bone marrow mesenchymal stem cel s group (cel group) injected with 1×107/kg bone marrow mesenchymal stem cel s via the left iliac vein (before peritoneal suture) and tail vein (from the 2nd day) for 10 days;bone marrow mesenchymal stem cel s+low/high dosage of curcumin groups (low/high dosage curcumin groups) injected intragastrical y with 2 or 10 mg/kg curcumin combined with injection of bone marrow mesenchymal stem cel s for 10 days. Transforming growth factor-β1 protein expression in the kidney tissues was examined by immunohistochemical staining. The concentrations of interleukin-2 and interleukin-6 in serum were detected by ELISA assay. <br> RESULTS AND CONCLUSION:After kidney transplantation, the protein expression of transforming growth factor-β1 in renal tubular epithelial cel s and renal interstitial cel s as wel as the concentrations of interleukin-2and interleukin-6 in serum were significantly higher in the transplantation group than the other groups (P<0.05). Compared with the cel transplantation group, the protein expression of transforming growth factor-β1 as wel as the concentrations of interleukin-2 and interleukin-6 reduced significantly in the low/high dosage curcumin groups (P<0.05). These findings indicate that simultaneous administration of curcumin and bone marrow mesenchymal stem cel s can effectively inhibit immune rejection reaction and improve renal function in rats after kidney transplantation.关键词
干细胞/骨髓干细胞/姜黄素/骨髓间充质干细胞/大鼠/肾移植/免疫耐受/免疫排斥/免疫调节/转化生长因子β1/白细胞介素2/白细胞介素6/广东省自然科学基金分类
医药卫生引用本文复制引用
张黎黎,吕军,宛霞,徐安平..姜黄素对骨髓间充质干细胞诱导肾移植免疫耐受的影响[J].中国组织工程研究,2015,(28):4450-4454,5.基金项目
广东省自然科学基金自由申请项目(S2012010009667)@@@@Funding:the Natural Science Foundation of Guangdong Province, No. S2012010009667 （S2012010009667）
