中国组织工程研究Issue(28)：4520-4525,6.DOI:10.3969/j.issn.2095-4344.2015.28.017
同种异体脂肪干细胞复合脱钙骨支架材料修复尺骨缺损：CT扫描及组织学检测
Allogeneic adipose-derived stem cells combined with demineralized bone matrix for repair of ulna defects:CT scan and histological detection
摘要
Abstract
BACKGROUND:Adipose-derived stem cel s have a wide variety of sources and strong proliferation ability, which are easy to access and simple to culture. Therefore, adipose-derived stem cel s that are secondary to bone marrow mesenchymal stem cel s are expected to become the most promising seed cel s for bone tissue engineering. <br> OBJECTIVE:To explore the feasibility of rabbit adipose-derived stem cel s with demineralized bone matrix to repair ulna defects. <br> METHODS:Ulna defect model was made in rabbits. Demineralized bone matrix was implanted into the right defect region as control group. After osteogenic induction, rabbit adipose-derived stem cel s/demineralized bone matrix composite was implanted into the left defect region as experimental group. At 12 weeks after implantation, defect tissues were taken for CT scanning and histological detection. <br> RESULTS AND CONCLUSION:CT results showed that there was unclear boundary between the broken ends of fractured bone and the composite material in the experimental group, and paral el cal uses out of the broken end could be seen. In the control group, the broken end was clearly seen and no cal us occurred continuously. Fibers were connected at the defect site, and no new bone occurred. Histological findings showed that typical regenerated bone tissues were seen in the experimental group with osteocytes, bone lacunae and bone trabeculae;there were more osteocytes and bone lacunae, but bone trabecula was only seen in a part of bone defects;a few of col agens interlarded the regenerated bone tissues. In the control group, the residual of demineralized bone matrix was seen as wel as some col agenous fibers, and periosteal bone formed a little, but no large amount of regenerated osteoid tissues were found. These findings indicate that under osteogenic induction, rabbit adipose-derived stem cel s combined with demineralized bone matrix are feasible to repair ulna defects.关键词
干细胞/脂肪干细胞/尺骨缺损/动物模型/骨修复/同种异体/脱钙骨分类
医药卫生引用本文复制引用
杨函,康建平,丁裕名,王松..同种异体脂肪干细胞复合脱钙骨支架材料修复尺骨缺损：CT扫描及组织学检测[J].中国组织工程研究,2015,(28):4520-4525,6.基金项目
四川医科大学自然科学基金(2013ZRQN071)@@@@Funding:the Natural Science Foundation of Sichuan Medical University, No.2013ZRQN071 （2013ZRQN071）
