烟草科技Issue(8)：15-19,5.DOI:10.16135/j.issn1002-0861.20150804
废弃烟梗提取液为基质的产油脂酵母菌的筛选与鉴定
Screening and Identification of Oleaginous Yeasts from Fermentation of Tobacco Stem Extract
摘要
Abstract
Using tobacco stem extract (TSE) as a sole substrate, 12 strains of oleaginous yeasts were preliminarily isolated from fresh grapes by Sudan Black B staining method. Two strains, named as CC820 and CA815, with higher lipid yield and growing well in TSE were screened out by further shake flask fermentation experiments. By their colony, morphological, physiological and biochemical characteristics, CC820 was preliminarily identified as Candida sp and CA815 Rhodotorula sp. When stem was fermented at 30 ℃ for 5 days with an inoculant rate of 5%, a load capacity of 60/250 mL and 180 r/min, the maximum yield of lipid produced by CC820 and CA815 reached 3.53 g/L and 3.29 g/L, respectively.关键词
烟梗/酵母菌/油脂/筛选/鉴定Key words
Tobacco stem/Yeast/Lipid/Screening/Identification分类
轻工纺织引用本文复制引用
王姣,李彦周,朱大恒,席宇,陈彦好,张向磊,梁景辉,刘慧,张旭萍,吴晨磊,董贵彬,丁腾阁..废弃烟梗提取液为基质的产油脂酵母菌的筛选与鉴定[J].烟草科技,2015,(8):15-19,5.基金项目
国家级大学生创新创业训练计划资助项目“废弃烟梗为基质的产油酵母菌的筛选鉴定及发酵产油优化研究”（201410459092）和“废弃烟梗固态发酵生产深红酵母的优化及扩大培养研究”（201310459009）；河南省烟草公司科技项目“烟草基质有机酸发酵与资源利用生物工程”（HYKJ201125）；河南省高等学校重点科研项目“干酪乳杆菌XJL发酵废弃烟梗水提取物产L-乳酸的响应面优化研究”（15B180020）。 （）
