医药导报Issue(8)：1067-1071,5.DOI:10.3870/yydb.2015.08.021
高效液相色谱法测定复方丹参片与滴丸中7种活性成分含量
Determination of Seven Active Components Compared with Fufang Danshen Tablets and Dripping Pills by HPLC
摘要
Abstract
Objective To establish a HPLC method for simultaneous determination of seven active components in Fufang Danshen tablets and Fufang Danshen dripping pills. Methods These seven compounds were analyzed simultaneously with a Zorbax C18 column by gradient elution using acetonitrile-0. 1% phosphoric acid solution as mobile phase, the flow rate was 1 mL·min-1 and the detection wavelength was set at 203, 270 and 281 nm, respectively. Results All the seven components showed good linear relation between peak area and concentration of the test, and the average recoveries were between 95. 1%-100. 4%. Tanshinone ⅡA was not detected in samples of dropping pills. Conclusion The HPLC method to determine the components including tanshinone ⅡA, salvianolic acid B, propanoid acid, protocatechuic aldehyde, notoginsenoside R1 , ginsenoside Rg1 and ginsenoside Rb1 of the two different Danshen preparations has been established, and it has the advantages of simplicity, high precision, good repeatability, and can be used for the quality control of two kinds of Fufang Danshen preparations. The content of tanshinone Ⅱ A in Fufang Danshen tablet was distinctly higher than that of dropping pills.关键词
丹参片,复方/丹参滴丸,复方/活性成分/色谱法,高效液相Key words
Fufang Danshen tablets/Fufang Danshen dripping pills/Active components/Chromatography, high performance liquid分类
医药卫生引用本文复制引用
张爱兵,张珺,程月发,张春雨,郭兰,刘颖硕..高效液相色谱法测定复方丹参片与滴丸中7种活性成分含量[J].医药导报,2015,(8):1067-1071,5.基金项目
唐山市2013年科学技术研究与发展计划基金资助项目(13130298z) （13130298z）
2012年河北联合大学科学研究基金资助项目(z201242) （z201242）
