中国病理生理杂志Issue(8)：1360-1364,5.DOI:10.3969/j.issn.1000-4718.2015.08.003
降钙素基因相关肽对血管平滑肌细胞心肌素表达及细胞表型改变的作用
Effect of calcitonin gene-related peptide on myocardin expression and phenotypic switch in vascular smooth muscle cells
摘要
Abstract
AIM:Toinvestigatetheeffectsofcalcitoningene-relatedpeptide(CGRP)onmyocardinexpres-sion and phenotypic switch in vascular smooth muscle cells ( VSMCs) .METHODS:VSMCs were obtained by aortic tissue adherent culture and treated with angiotensin Ⅱ( AngⅡ) , AngⅡ+CGRP or AngⅡ+CGRP +CGRP8-37 .The protein expression of myocardin and the phenotypic proteins of the VSMCs was detected by Western blot .RESULTS:The expres-sion of myocardin in cultured VSMCs showed downregulation along with time expansion .The protein level of myocardin was higher at 48 h and 72 h than that at baseline in the cultured VSMCs (P<0.05).However, the myocardin was lower at 48 h and 72 h than that at baseline after treatment with CGRP in cultured VSMCs (P<0.05).Furthermore, at 48 h in cul-tured VSMCs, the myocardin decreased along with α-smooth muscle actin (α-SMA) (P<0.05), and osteopontin (OPN) increased (P<0.05) in AngⅡ group compared with control group .After treatment with CGRP, the levels of myocardin andα-SMA become higher ( P <0.05 ) but OPN was lower ( P <0.05 ) in CGRP group than those in AngⅡ group. CGRP8-37 abrogated CGRP-induced increase in myocardin and α-SMA and decrease in OPN in CGRP 8-37 group compared with CGRP group .CONCLUSION: CGRP may regulate the phenotypic switch of the VSMCs and maintain the cells in contractile phenotype through the upregulation of myocardin protein , which may be accomplished by the combination of CGRP and its receptor .关键词
血管平滑肌细胞/降钙素基因相关肽/心肌素/表型改变Key words
Vascularsmoothmusclecells/Calcitoningene-relatedpeptide/Myocardin/Phenotypicswitch分类
医药卫生引用本文复制引用
龙仙萍,邓文文,汪松,王冬梅,盛瑾,石蓓,赵然尊..降钙素基因相关肽对血管平滑肌细胞心肌素表达及细胞表型改变的作用[J].中国病理生理杂志,2015,(8):1360-1364,5.基金项目
国家自然科学基金资助项目（No．81360021）；贵州省国际合作项目［黔科合外G字（2013）7037号］ （）
