中国病理生理杂志Issue(8)：1412-1416,5.DOI:10.3969/j.issn.1000-4718.2015.08.011
千金藤素诱导人肺腺癌 LTEP－a－2细胞中miRNA 表达变化的研究
Cepharanthine induces miRNA expression in human lung adenocarcinoma LTEP-a-2 cells
摘要
Abstract
AIM:ToobservetheeffectofcepharanthineonhumanlungadenocarcinomaLTEP-a-2cellgrowth, and to explore the changes of related microRNA ( miRNA) expression in the cells .METHODS:LTEP-a-2 cells were trea-ted with cepharanthine at concentrations of 0μmol/L, 10μmol/L, 20μmol/L and 40μmol/L.The growth inhibition rate was detected by MTT assay , and the cell morphological changes were observed under light microscope .The cell apoptosis was analyzed by flow cytometry .The expression of let-7c, miR-34a and miR-34b was measured by real-time PCR.RE-SULTS:Cepharanthine inhibited the cell activity of LTEP-a-2 cells in a dose-dependent manner .With the increase in cepharanthine concentration , the pyknosis of the cells was visible under the inverted microscope .Flow cytometry analysis found that different concentrations of cepharanthine induced the increase in the apoptotic rates of LTEP -a-2 cells.The re-sults of real-time PCR showed that the cepharanthine also increased the expression of let -7c, miR-34a and miR-34b.CON-CLUSION:Cepharanthine inhibits the growth of LTEP-a-2 cells, and induces apoptosis .Cepharanthine increases the ex-pression of let-7c, miR-34a and miR-34b, indicating that these miRNAs in LTEP-a-2 cells has the function as tumor sup-pressor genes .关键词
千金藤素/LTEP-a-2细胞/微小RNAKey words
Cepharanthine/LTEP-a-2cells/MicroRNA分类
医药卫生引用本文复制引用
张亚龙,张菡菡,阎云飞,庞敏,谢书阳,李有杰..千金藤素诱导人肺腺癌 LTEP－a－2细胞中miRNA 表达变化的研究[J].中国病理生理杂志,2015,(8):1412-1416,5.基金项目
国家自然科学基金资助项目（ No．31371321）；山东省自然科学基金资助项目（ No．ZR2013HL003；No． ZR2014HQ079）；山东省高校科技计划资助项目（No．J13LE11）；滨州医学院科技计划项目 （）
