中国病理生理杂志Issue(8)：1467-1471,5.DOI:10.3969/j.issn.1000-4718.2015.08.021
脊髓背角MCP－1－JAK2／STAT3信号转导参与大鼠2型糖尿病神经病理性痛的机制研究
Mechanism of MCP-1-JAK2/STAT3 signal transduction in spinal cord dorsal horn contributing to rats with type 2 diabetic neuropathic pain
摘要
Abstract
AIM:ToexplorewhetherMCP-1-JAK2/STAT3signaltransductioninthespinaldorsalhornin-volves the formation and development of rat type 2 diabetic neuropathic pain (DNP).METHODS: The male Sprague-Dawley rats were fed with a high-fat and fructose diet for 8 weeks,and then received a single intraperitoneal streptozocin in-jection to prepare the type 2 DNP model.The type 2 DNP rats were randomly divided into 4 groups (n=16):DNP group, MCP-1 neutralizing (DM) group, DNP+AG490 (DA) group and solvent control (SC) group.A catheter of PE-10 was placed into the subarachnoid space of the rats in groups DM , DA and SC.After 3 d, the rats in DM,DA and SC groups were injected with MCP-1 inhibitor 10μL at 0.1 mg/L, AG490 10μL at 1 mmol/L and DMSO 10μL at 3.5%once a day for 14 days, respectively.Another 16 normal rats were selected as control (C) group and were fed with common forage. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured at 1, 3, 7 and 14 d after subarachnoid injection .The lumbar segments 4-6 of the spinal cord were removed at the same time for determination of the expressions of p-JAK2 and p-STAT3 by Western blot .RESULTS:Compared with C group , MWT was significantly de-creased, TWL was shortened and the expression of p-JAK2 and p-STAT3 in the spinal dorsal horn was up-regulated at 1, 3, 7 and 14 d in DNP and SC groups (P<0.05).Compared with DNP group, MWT was significantly increased, TWL was prolonged and the expression of p-JAK2 and p-STAT3 in spinal dorsal horn was down-regulated at 1, 3, 7 and 14 d in DM and DA groups (P<0.05).No significant difference in the MWT, TWL and expression of p-JAK2 and p-STAT3 between DNP group and SC group was observed (P>0.05).CONCLUSION:The MCP-1-JAK2/STAT3 signal transduction in the spinal dorsal horn involves the formation and development of DNP in rats .关键词
2型糖尿病/脊髓背角/单核细胞趋化蛋白1/Janus激酶2/信号转导及转录激活因子3Key words
Type2diabetes/Spinaldorsalhorn/Monocytechemoattractantprotein-1/Januskinase2/Signal transducer and activator of transcription 3分类
医药卫生引用本文复制引用
袁超,管金川,曹红,李军..脊髓背角MCP－1－JAK2／STAT3信号转导参与大鼠2型糖尿病神经病理性痛的机制研究[J].中国病理生理杂志,2015,(8):1467-1471,5.基金项目
国家自然科学基金资助项目（No．81073125）；浙江省自然科学基金资助项目 （）
