中国康复理论与实践Issue(8)：900-904,5.DOI:10.3969/j.issn.1006-9771.2015.08.006
电针曲池、足三里穴对缺血再灌注损伤大鼠线粒体Caspase-3途径诱导细胞凋亡的影响
Effects of Electroacupuncture at Quchi (LI11) and Zusanli (ST36) on Neuronal Apoptosis Induced by Mitochon-dria-caspase-3 Pathway in Rats with Cerebral Ischemia-reperfusion Injury
摘要
Abstract
Objective To investigate the effects of electroacupuncture (EA) at Quchi (LI11) and Zusanli (ST36) acupoints on the ultra-structural structure of cortical neurons in peripheral area and the protein expression of caspase-3, Bcl-2, Bax in rats with cerebral isch-emia-reperfusion injury. Methods 36 male adult Sprague-Dawley rats were randomly divided into sham operation group, model group, and electroacupuncture group, with 12 rats in each group. The model group and electroacupuncture group were performed with left middle cere-bral artery occlusion (MCAO) according to the modified Longa' methods. The electroacupuncture group received electroacupuncture at Qu-chi (LI11) and Zusanli (ST36) on the paralyzed limb, for 30 minute. The neurobehavioral scores were recorded before and after treatment. The ultrastructural structure of cortical neurons was observed with transmission electron microscope (TEM). The protein expression of cas-pase-3, Bcl-2 and Bax were detected by Western blotting technique. Results The neurobehavioral score was lower in the electroacupuncture group than in the control group (P<0.05). Compared with the model group, the chromatin of neurons was even relatively, and the number of mitochondria increased. The expression of Bcl-2 was higher and the expression of caspase-3 and Bax was lower in the electroacupuncture group than in the model group (P<0.05). Conclusion Electroacupuncture at Quchi (LI11) and Zusanli (ST36) acupoints can inhibit the neu-rons apoptosis in peripheral area through mitochondria-caspase-3 pathway.关键词
脑缺血再灌注/电针/线粒体/caspase-3/凋亡/大鼠Key words
cerebral ischemia-reperfusion/electroacupuncture/mitochondria/caspase-3/apoptosis/rats分类
医药卫生引用本文复制引用
上官豪,柳维林,陈文列,郑薏,王鲜,林云娇,王露露,陈立典,陶静..电针曲池、足三里穴对缺血再灌注损伤大鼠线粒体Caspase-3途径诱导细胞凋亡的影响[J].中国康复理论与实践,2015,(8):900-904,5.基金项目
国家自然科学基金项目(No.81273835 （）
No.81373778)。 （）
