中国康复理论与实践Issue(8)：905-912,8.DOI:10.3969/j.issn.1006-9771.2015.08.007
神经酰胺通过JNK/c-Jun信号通路诱导胶质瘤细胞自噬性死亡
Autophagic Cell Death in Glioma Cell Induced by Ceramide through JNK/c-Jun Pathway
张露勇 1张淼 2刘师卜 1李锐3
作者信息
- 1. 中国食品药品检定研究院食品化妆品所，北京市100044
- 2. 中国人民解放军总医院外科药房，北京市100853
- 3. 中日友好医院神经外科，北京市100029
- 折叠
摘要
Abstract
Objective To observe the autophagy of 87-MG and U251 glioma cells induced by ceramide and explore the possible mecha-nism. Methods The viability and apoptosis of 87-MG and U251 cells were detected by MTT assay and flow cytometry, respectively. Autoph-agic-related protein expressions of LC3B/LC3A and Beclin-1 were determined by Western blotting. The activation of JNK/c-Jun signaling pathway induced by ceramide with or without the treatment of JNK specific inhibitor SP600125 was also measured. Results 24 hours after treatment of ceramide, the growth of 87-MG and U251 cells was significantly inhibited time-dependently (P<0.05);and the number of au-tophagic cells increased dose-dependently (P<0.05). The levels of LC3B/LC3A and Beclin-1 significantly increased after ceramide treat-ment (P<0.05). JNK signaling pathway was activated in the 87-MG and U251 cells and the phosphorylation of c-Jun also increased after ce-ramide treatment. This activation of autophagy could be reversed by the pre-treatment of SP600125. Conclusion Ceramide may induce au-tophagy in 87-MG and U251 glioma cells and the mechanism may be related to the activation of JNK/c-Jun signaling pathway.关键词
胶质瘤/神经酰胺/自噬性死亡/JNK信号通路Key words
glioma cell/ceramide/autophagic cell death/JNK signaling pathway分类
医药卫生引用本文复制引用
张露勇,张淼,刘师卜,李锐..神经酰胺通过JNK/c-Jun信号通路诱导胶质瘤细胞自噬性死亡[J].中国康复理论与实践,2015,(8):905-912,8.
