中国免疫学杂志Issue(8)：1019-1023,5.DOI:10.3969/j.issn.1000-484X.2015.08.003
CCR3在哮喘小鼠气道组织Muc5 ac表达中的作用
Effects of CCR3(Chemokine receptor-3) on Muc5ac in airway of asthmatic mice
摘要
Abstract
Objective:To investigate the effects of CCR3 on Muc5ac in the airway of asthmatic mice.Methods: Fifty clean BALB/c mice were randomly divided into control group,asthmatic group,dexamethasone treatment group,SB328437 treatment group, vehicle-control group.Total cells and differential inflammatory cells were counted in BALF, the levels of IL-4 and TNF-αwere determined by ELISA,lung tissue HE staining the expressions of Mucin5ac(Muc5ac) and CCR3 in lung tissue were detected by immu-neohistochemical staining and RT-PCR.Results:The total cells,eosinophil,monocytes and lymphocyte cells in BALF,the levels of IL-4,TNF-αin BALF ,the goblet cell of airway wall,the expression of Muc5ac and CCR3 positive staining IOD in the airway and the expression of Muc5ac and CCR3 mRNA lung tissue obviously decreased in SB328437 treatment group and DEX treatment group which compared with asthmatic mice model group ( P<0.05 ) .Conclusion: SB328437 can inhibit the expression of CCR3 in pulmonary tissue,furtherly inhibit the expression and secretion of Muc5ac and control the airway inflammation.关键词
哮喘/小鼠/黏蛋白5ac(Muc5ac)/趋化因子受体3拮抗剂Key words
Asthma/Mouse Mucin5ac (Muc5ac)/Chemokine receptor-3 antagonist分类
医药卫生引用本文复制引用
王涛,张建勇,王秀丹,郑飞彦,杨红霞..CCR3在哮喘小鼠气道组织Muc5 ac表达中的作用[J].中国免疫学杂志,2015,(8):1019-1023,5.基金项目
本文为贵州省科学技术基金项目(黔科合 J 字［2007］2018号）和贵州省科技厅基金项目(黔科合SY字［2008］3065）。 （）
