中国免疫学杂志Issue(8)：1060-1065,6.DOI:10.3969/j.issn.1000-484X.2015.08.013
重组BCG的抑瘤活性及免疫学机制研究
Antitumor activity and immunological mechanisms of recombinant BCG
摘要
Abstract
Objective:To study the antitumor activity and immunological mechanisms of rBCG including GM-CSF and EB virus LMP2A gene fusion.Methods: Animal models of EB virus-positive tumors was built.The formation time of tumors in mice,survival time,tumor weight was analyzed to detect rBCG anti-tumor activity;ELISA method was used to detect the specific antibodies which was produced in the mice stimulated by rBCG,specific CTL killing effect was detected by lactic dehydrogenase assay,ELISPOT was used to assay the secretion of IFN-γand flow cytometry, HE staining of tumor tissue was used to detected lymphocyte infiltration in mice immunized with recombinant BCG.Statistical methods were used for rBCG immunization effect preliminary analysis and evaluation.Results:Comparing to other control,tumor formation time was significantly delayed and tumor growth was slow, survival time of mice prolonged .ELISA test results showed that the rBCG immunization groups of mice could produce specific IgG antibodies of GM-CSF and LMP2A.Specific CTL activity was detected in mice immunized with rBCG.IFN-γsecretion was detected by ELISPOT method, flow cytometry and morphological observation detected tumor tissue infiltration of lymphocytes growth in mice immunized with rBCG.Conclusion:The rBCG induced a humoral and cellular immune responses and induced C57BL/6 mice to produce a strong anti-tumor effect and the EB virus-positive tumor cells was significantly inhibited.关键词
重组BCG/抗肿瘤/细胞免疫/EB病毒Key words
rBCG/Anti-tumor/Cellular immune/EB virus分类
医药卫生引用本文复制引用
薛庆节,李秀真,李运清,杨媛媛,胡文洁,章洪华,吕厚东,李士根..重组BCG的抑瘤活性及免疫学机制研究[J].中国免疫学杂志,2015,(8):1060-1065,6.基金项目
本文为山东省自然科学基金项目(ZR2012HM037）、山东省高等学校科技计划项目(No.J12LK56，J11LF03）和教育部留学回国人员科研启动基金资助项目(20101174）。 （）
