中国免疫学杂志Issue(8)：1066-1069,4.DOI:10.3969/j.issn.1000-484X.2015.08.014
过表达ALEX1对乳腺癌MCF-7细胞增殖和凋亡的影响
Effect of overexpression ALEX1 on proliferation and apoptosis
摘要
Abstract
Objective:To investigate the effects of ALEX1 overexpression on cell proliferation and apoptosis of human breast cancer cell line MCF-7.Methods: MCF-7 cells were infected recombinant lentivirus LV5-ALEX1 and the negative control lentivirus LV5-NC,respectively.After 72 h, the expression of ALEX1 was detected by Real-Time PCR and Western blot.CCK8 assay were performed to observe the proliferation ability after 24 h, 48 h, 72 h, 96 h.The effect of overexpression ALEX1 on apoptosis was determined by flow cytometry.The level of Bax,BCL-2 and active caspase3 was detected by Western blot.Results:The mRNA level of ALEX1 markedly increased after 72 h(165.81±12.14 vs 52.29±2.32,P<0.01).In CCK8 assay,the results revealed that the cell pro-liferation was inhibited compared with control group in 48 h,72 h,96 h( P<0.05).The results revealed that overexpression of ALEX1 enhanced MCF-7 apoptosis(20.55%±2.50 % vs 3.60%±1.614%,P<0.05).The results by Western blot showed that the protein levels of Bax and active caspase were increased in LV5-ALEX1 group compared with LV5-NC group.However,the protein levels of BCL-2 was decreased in LV5-ALEX1 group compared with LV5-NC group.Conclusion:Overexpression of ALEX1 significantly reduced MCF-7 cancer cells proliferation and induced MCF-7 cells apoptosis.关键词
乳腺癌/MCF-7细胞/细胞增殖/凋亡Key words
Breast cancer/MCF-7 cells/Cell proliferation/Apoptosis分类
医药卫生引用本文复制引用
曾帆,高月,伍家燕,李海玉,樊建军,李韵,张寒韬,刘革力,宋方洲..过表达ALEX1对乳腺癌MCF-7细胞增殖和凋亡的影响[J].中国免疫学杂志,2015,(8):1066-1069,4.基金项目
本文为高等学校博士学科点专项科研基金资助课题(20125503110012）。 （）
