中国全科医学Issue(23)：2817-2821,5.DOI:10.3969/j.issn.1007-9572.2015.23.017
钩藤碱治疗骶上脊髓损伤致逼尿肌反射亢进大鼠的实验研究
Experimental Study of the Effect of Phynchophylline on Detrusor Overactivity Induced by Sacral Spinal Cord Injury in Rats
摘要
Abstract
Objective To investigate the effects of phynchophylline on detrusor overactivity induced by sacral spinal cord injury ( SSCI) in rats and the underlying mechanism .Methods Using random number table method , we divided 30 10-week female healthy SD rats into control group , model group and treatment group , with 10 rats in each group.No surgical intervention was made in control group , neurogenic bladder models were made in model group and treatment group by spinal cord transaction.Control group and model group were administrated with 0.9%sodium chloride solution by intraperitoneal infection . Treatment group was administrated with phynchophylline (5 mg/kg).10 rats in control group were all alive, successful model building was made in 8 rats in model group and 8 rats in treatment group . Four weeks later , urine dynamics testing was undertaken with the bladder of rats , in vitro test of the electrophysiological indicators of detrusor was conducted , the pathological sections of bladder were observed by HE staining method , c-kit protein expression was tested by western blotting method , the change in the number of ICC was observed under laser scanning confocal microscope , and the expression level of α1G gene of subunit of T-type calcium channels in ICC was detected by real -time PCR.Results Compared with the model group , the treatment group had larger maximum capacity of bladder (q=8.251, P<0.05), lower bladder leak point pressure (q=3.764, P<0.05), lower bladder filling pressure (q =5.687, P <0.05), lower detrusor contraction frequency (q =9.661, P <0.05), higher minimum tension (q=5.217, P<0.05), lower expression level of c -kit protein (q=13.688, P<0.05), lower number of ICC (q =7.060, P <0.05) and lower α1G gene expression (q =8.762, P <0.05).Conclusion Rhynchophylline can reduce the number of ICC cells in neurogenic detrusor and inhibit the pacemaker activity of ICC cells by inhibiting the expression of α1G gene of subunit of T -type calcium channels in ICC , thereby reducing excessive detrusor hyperactivity and make the excitability of detrusor become normal .关键词
逼尿肌过度活动/膀胱,神经原性/钩藤碱/Cajal间质细胞/钙通道, T型/α1G基因Key words
Overactive detrusor/Urinary bladder , neurogenic/Phynchophylline/ICC/Calcium channels , T -type/α1G gene分类
医药卫生引用本文复制引用
刘仁杰,姜华茂,何春秀..钩藤碱治疗骶上脊髓损伤致逼尿肌反射亢进大鼠的实验研究[J].中国全科医学,2015,(23):2817-2821,5.基金项目
辽宁省科技厅计划项目 （）
