中国全科医学Issue(24)：2934-2938,2945,6.DOI:10.3969/j.issn.1007-9572.2015.24.013
bcr／abl基因特异性小干扰 RNA 对 K562细胞增殖凋亡和 SH2肌醇磷脂酶基因表达的影响研究
Effects of siRNA Against bcr/abl on Expression of SHIP and the Proliferation and Apoptosis of K562
摘要
Abstract
Objective Through silence the expression of bcr/abl with siRNA to increase the expression of SHIP so that we can study the influence of SHIP gene expression to the proliferation and apoptosis of K 562 together with its possible mechanism.Methods Construct synthetic siRNA specific to bcr/abl fusion gene and tranfect it into cell line K 562, non -specific siRNA trasfected group and untreated group were used as control , then Western blotting was employed to detect the expression of p210, SHIP and level of p -Akt (308, 437), SHIP mRNA level was detected through FQ -RT -PCR, proliferation of K562 through MTT, and apoptosis of K562 through flow cytometre, NF-κB activity was examined through ELISA.Results fter transfected with siRNA specific to bcr/abl, the expression of p210, p -Akt308, p -Akt473 was dramatically decreased in the bcr -abl-specific siRNA transfected group compare to the non -specific transfected control and untreated group of K562 cells, while the SHIP mRNA, protein expression, apoptosis rate increased in bcr -abl -specific transfected K562 cells compare to the other two control groups (P<0.05).All these changes were not detected between the non-specific transfected group and untreated group of K 562 cells (P>0.05).The proliferation decreased in the bcr -abl-specific transfected group compare to the other two controls on day 1, day 3 and day 5 respectively (P<0.05), but no significance was found between the two control groups (P>0.05).NF-κB activity decreased in bcr -abl-specific transfected group compare to the other two control groups on day 3 and day 5 ( P<0.05) , but no significance was found between the two control groups ( P>0.05).Conclusion Our results suggested that SHIP expression is controlled by bcr /abl.Reduced expression of SHIP partly responsible for the increased proliferation and decreased apoptosis of bcr /abl positive cells .关键词
白血病/基因, bcr/abl/RNA, 小分子干扰/细胞增殖/细胞凋亡Key words
Leukemia/Genes, bcr/abl/RNA, small interfering/Cell proliferation/Apoptosis分类
医药卫生引用本文复制引用
刘小军,杨琳,潘玉夏,王兴哲,尚银涛,王茜,杨敬慈,罗建民..bcr／abl基因特异性小干扰 RNA 对 K562细胞增殖凋亡和 SH2肌醇磷脂酶基因表达的影响研究[J].中国全科医学,2015,(24):2934-2938,2945,6.基金项目
国家自然科学基金资助项目（30240011）；河北省自然科学基金资助项目（2007000858）；卫生行业科研专项基金 （）
