中国蔬菜Issue(8)：22-27,6.
番茄品种SSR标记鉴定技术研究
Studies on Tomato Variety Identification Technology Using SSR Marker
摘要
Abstract
Taking 16 tomato hybrid varieties as material, 20 pairs of primer suited for tomato variety identification were screened from 200 pairs of SSR primer, and 66 tomato varieties were analyzed. 52 allelic gene variations were identified among these varieties. The allelic gene variation was in the range of 2-5, with an average of 2.6 allelic gene variation each locus. The cultivar identification ability of primer combination was analyzed by genetic clustering method. The results showed that the 66 tomato varieties could be efficiently identified by only 9 pairs of SSR primer. The SSR-DNA fingerprinting constructed with these 9 pairs of primer combination could be used as genetic tag for variety authenticity identification.关键词
番茄/SSR/品种鉴定/指纹图谱Key words
Tomato/SSR/Variety identification/Fingerprinting引用本文复制引用
赵海艳,吴明生,宋歌,牛茜,田慧芳..番茄品种SSR标记鉴定技术研究[J].中国蔬菜,2015,(8):22-27,6.基金项目
北京市科技计划项目 （）
