中国药房Issue(22)：3089-3091,3.DOI:10.6039/j.issn.1001-0408.2015.22.20
磷脂酰肌醇3-激酶抑制剂LY29400联合吲哚-3-甲醇对人甲状腺未分化癌细胞FRO增殖的抑制作用
Inhibitory Effects of Phosphatidylinositol 3-kinase Inhibitor LY29400 Combined with Indole-3-carbinol on the Proliferation of Human Anaplastic Thyroid Carcinoma FRO Cells
王文斌 1刘杨 1黄明 1陈大平 1任平2
作者信息
- 1. 咸宁市中心医院甲乳外科，湖北咸宁 437100
- 2. 湖北科技学院药学院，湖北咸宁 437100
- 折叠
摘要
Abstract
OBJECTIVE:To study the inhibitory effects of phosphatidylinositol 3-kinase (PI3K) inhibitor LY29400 (“LY”) combined with indole-3-carbinol (I3C) on the proliferation of human anaplastic thyroid carcinoma FRO cells. METHODS:RFO cells in logarithmic growth phase were divided into blank control group,I3C (250 µmol/L) group,LY (10 µmol/L) group and combination (I3C of 250 µmol/L + LY of 10 µmol/L) group,which were subject to 24,48 and 72 h drug action. MTT method was used to determine and calculate the inhibitory rates of the cells in all groups,flow cytometry assay to determine the apoptosis rates after 48 h action,Western blot method to determine the expressions of Capspase-3 proteins thereafter and immunohistochemi-cal method to determine the expressions of Bcl-2 and Bax thereafter. RESULTS:The inhibitory rate obviously increased with the ex-tension of drug action time,with statistical significance at each time point in combination group (P<0.05). Compared to I3C group and LY group,combination group had higher inhibitory rate,apoptosis rate and the expressions of Capspase-3 protein and Bax,and lower Bcl-2 expression and ratio of Bcl-2 to Bax,with statistical significance (P<0.01). CONCLUSIONS:LY com-bined with I3C has synergistic inhibitory effects on the proliferation of FRO cells by a mechanism which may be related to apopto-sis induced by down-regulating Bcl-2 expression,up-regulating Bax expression and activating Caspase cascade reaction.关键词
磷脂酰肌醇3-激酶抑制剂/吲哚-3-甲醇/人甲状腺未分化癌细胞FRO/增殖抑制率/细胞凋亡Key words
Phosphatidylinositol 3-kinase inhibitor/Indole-3-carbinol/Human anaplastic thyroid carcinoma FRO cells/Inhibito-ry rate/Apoptosis分类
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王文斌,刘杨,黄明,陈大平,任平..磷脂酰肌醇3-激酶抑制剂LY29400联合吲哚-3-甲醇对人甲状腺未分化癌细胞FRO增殖的抑制作用[J].中国药房,2015,(22):3089-3091,3.
