中国药房Issue(22)：3109-3111,3112,4.DOI:10.6039/j.issn.1001-0408.2015.22.26
代谢组学法考察金银花醇提物对模型小鼠肝损伤的预防作用
Observation of Preventive Effect of Lonicera japonica Alcohol Extract on Mice with Liver Injury by Metab-olomics Method
罗时旋 1赵稷 1张宇 1孙长海 1江德田 1高金波 1滕杨1
作者信息
- 1. 佳木斯大学药学院，黑龙江佳木斯 154007
- 折叠
摘要
Abstract
OBJECTIVE:To study the preventive effect of Lonicera japonica alcohol extract on mice with liver injury based on metabolomics method. METHODS:30 mice were equally randomized into a normal control (isometric normal saline) group,a model(isometric normal saline)group and a group of L. japonica alcohol extract(2 g/kg). The mice were given drugs by ig once a day for 14 consecutive days. On the 8th day of administration,the models were established by giving 0.2% dimethyl sulfoxide (DMN,10 ml/kg)ip once a day for 7 consecutive days. Gas chromatography-mass spectrum(GC-MS)was used to analyze 24 h total ions chromatogram of the urine sample on the 1st,3rd,5th and 7th day of administration of drugs and DMN. The change in endogenic small molecule metabolites in urine was observed. Principal component analysis was employed to explore the change in the metabolite chromatogram and underlying biomarkers in urine. RESULTS:The contour of the chromatogram changed to a largest extent 1 to 5 d after given DMN,but showed an obvious trend towards regression 7 days thereafter. DMN resulted in increase in the contents of 8-phenyl-8-azbicyclo-[4,3,0]non-3-ene-7,9-dione,2-(6-heptynyl)-1,3 dioxolane,bis-(O-methyloxime)-4-ketoglu-cose,and decrease in the contents of malonic acid,2-(4- chlorophenylthiomethoxyl)ethyl,tetrahydro-2-furanacetaldehyde,D-ga-lactose,erythro-pentonic acid and galacturonic acid,in endogenic small molecule metabolites in mouse urine,for which Lonicera japonica alcohol extract can improve that. CONCLUSIONS:Previous administration of L. japonica alcohol extract ig has preven-tive effect to some extent on the physiological and metabolic conditions of mice with liver injury induced by DMN.关键词
金银花醇提物/代谢组学/肝损伤/小鼠Key words
Lonicera japonica alcohol extract/Metabolomics/Liver injury/Mice分类
医药卫生引用本文复制引用
罗时旋,赵稷,张宇,孙长海,江德田,高金波,滕杨..代谢组学法考察金银花醇提物对模型小鼠肝损伤的预防作用[J].中国药房,2015,(22):3109-3111,3112,4.
