中国药理学通报Issue(8)：1085-1090,1091,7.DOI:10.3969/j.issn.1001-1978.2015.08.012
朱槿花苞提取物治疗皮肤疖痈溃疡及其机制研究
Research in skin healing and repair function and mechanism of Hibiscus rosa-sinensis Linn bud extract
摘要
Abstract
Aim To test the skin healing and repairing efficacy and the mechanism of Hibiscus rosa-sinensis L bud extract by using the animal models. Methods KM mice were randomly divided into three groups:the model group, the positive control group, and the n-bu-tyl alcohol extract ( HrBN) group. Using the boils and carbuncles model, the healing condition of all the animals were observed. KM mice were kept in the SPF condition room and divided into five groups: the model group, the positive control group, and the low, middle, high dose groups. Using the full-thickness loss model, the repairing results of all the mice were ob-served. Through the antimicrobial test, the results of MIC and inhibition zone were obtained. The carbon clearance test was used to collect the blood at the time 5min and 15min, and get the liver and spleen, and the results of K andαwere obtained. Results In vivo ex-periments showed there was significant difference be-tween groups;the HrBN extract had the outstanding ef-ficacy in healing and repairing skin boils and full-thickness loss models. It had higher recovery rate than other ethanol extract, such as ethyl acetate extract and chloroform extract. In vitro experiments showed that the HrBN extract, ethyl acetate extract ( HrBE) ,AB-8 macroporous resin 30% alcohol part and 60% alcohol part had obvious antimicrobial efficacy. The carbon clearance test showed HrBN had a good effect in im-proving immune function, and it can increase the K and α. Conclusion HrBN in animal models exerts good skin healing and repairing efficacy, which might be related to its antibacterial activity and immunologic enhancement function.关键词
朱槿花苞/正丁醇萃取物/疖痈模型/全层缺失模型/抗菌/碳粒廓清Key words
Hibiscus rosa-sinensis Linn bud/n-butyl alcohol extract/boils and carbuncles model/full-thick-ness loss model/antimicrobial/carbon clearance test分类
医药卫生引用本文复制引用
姜霁洋,徐涵,白雪婷,程訸,林艳玲,凌震,陈一村,石刚刚..朱槿花苞提取物治疗皮肤疖痈溃疡及其机制研究[J].中国药理学通报,2015,(8):1085-1090,1091,7.基金项目
广东省自然科学基金-团队项目( No 9351503102000001) （ No 9351503102000001）
中央财政支持地方高校发展专项资金 （）
