中国药理学通报Issue(8)：1136-1141,6.DOI:10.3969/j.issn.1001-1978.2015.08.022
RDP多肽修饰的姜黄素隐形脂质体脑靶向作用的研究
Brain targeting effect of PEGylated liposomes modified with RDP peptide
摘要
Abstract
Aim Targeted drug delivery in the brain is the necessary way for the treatment of brain diseases. In our study, a new peptide derived from the rabies vi-rus glycoprotein ( RVG-derived peptide, RDP ) was used as a targeted carrier to modify the curcumin stealth liposomes, and their characteristics and brain targeting effect were studied. Methods The curcumin liposomes were prepared by thin film dispersion. The release test in vitro was conducted to investigate their drug release. Curcumin distribution in several organs of mice was investigated by caudal vein injection of curcumin suspension liquid ( CUR ) , curcumin lipo-somes ( CUR-L) , RDP modified curcumin stealth lipo-somes ( RDP-CUR-L) via HPLC assay at different time points. Results The prepared stealth nano liposomes had a size of around 100 nm, and also had a good dis-persion and reproducibility. The entrapment efficiency was larger than 85%. After caudal vein injection of CUR, CUR-L and RDP-CUR-L in mice respectively, no curcumin was detected in brain of CUR group, and only a little was detected in CUR-L group. Neverthe-less, high concentration of curcumin was detected in RDP-CUR-L group. Conclusion RDP can deliver li-posome into the brain, which may provide a new meth-od for the treatment of brain diseases.关键词
姜黄素/RDP/脂质体/体内分布/细胞穿膜肽/脑靶向Key words
curcumin/RDP/liposomes/biodistribu-tion/cell-penetrating peptides/brain targeting分类
医药卫生引用本文复制引用
项松涛,赵明,史现勋,付爱玲..RDP多肽修饰的姜黄素隐形脂质体脑靶向作用的研究[J].中国药理学通报,2015,(8):1136-1141,6.基金项目
国家自然科学基金资助项目( No 81273416) （ No 81273416）
教育部高校基本科研业务费(No XDJK2013A030) （No XDJK2013A030）
教育部回国人员启动基金[教外司留(2012)940号] （2012）
教育部新世纪优秀人才支持计划资助 （）
