中国药理学通报Issue(8)：1165-1168,4.DOI:10.3969/j.issn.1001-1978.2015.08.027
人C1 q/TNFα相关蛋白-6的表达、纯化及生物活性分析
Expression, purification and biological activities assay of human C1q and tumor necrosis factor related protein-6
摘要
Abstract
Aim To prepare soluble human C1 q and tumor necrosis factor related protein-6 in Escherichia coli and analyze the bioactivity. Methods Recombi-nant plasmid was transformed into E. coli expression strain, and the recombinant protein Trx-hCTRP6 was expressed induced by IPTG and then purified. Results Trx-hCTRP6 was expressed efficiently and purified using Ni-NTA affinity chromatography and Superdex G-75 column. The purified Trx-hCTRP6 was shown to be active under in vivo and in vitro assay conditions. Con-clusion Active Trx-hCTRP6 is efficiently prepared from E. coli protein expression system.关键词
C1q/TNFα相关蛋白-6/大肠杆菌/重组蛋白/亲合纯化/蛋白印迹/分子筛Key words
C1 q and tumor necrosis factor related pro-tein-6/Escherichia coli/recombinant protein/affinity purification/Western blot/gel filtration chromatogra-phy分类
医药卫生引用本文复制引用
李洪波,高学飞,李娜,吴东海..人C1 q/TNFα相关蛋白-6的表达、纯化及生物活性分析[J].中国药理学通报,2015,(8):1165-1168,4.基金项目
国家自然科学基金资助项目( No 81300655) （ No 81300655）
湖南省重点学科建设项目( No 2011042) （ No 2011042）
广东省科技计划项目( No 2013B051000090) （ No 2013B051000090）
