中国医学创新Issue(22)：56-57,58,3.DOI:10.3969/j.issn.1674-4985.2015.22.021
密度梯度离心联合上游法的精液处理在供精体外受精胚胎移植中的应用
Application of Semen Preparation by the Density Gradient Centrifugation Technique Combined with the Swim- Up Method in Vitro Fertilization and Embryo Transfer with Donor Semen
摘要
Abstract
Objective:To observe embryonic development by using sperm selection prepared by the density gradient centrifugation technique combined with the swim-up method in vitro fertilization and embryo transfer with donor semen(D-IVF). Method:135 cases of routine D-IVF were retrospective analyzed from January 2013 to July 2014. The sperm condense,total activity rate and progressive motility were observed after sperm recovery and sperm selection. The fertilization rate,cleavage rate, high-quality embryo rate and pregnancy rate were compared by the 135 cases and the control groups consist of 46 cases of ICSI (intracytoplasmic sperm injection)with husband semen. Result:After treatment,the total activity rate of sperm and the sperm activity rate were significantly increased,but the sperm density was significantly decreased,the differences were statistically significant(P<0.05).Compared the fertilization rate,cleavage rate,high quality embryo rate and pregnancy rate of two groups, the differences were no statistically significant(P>0.05).Conclusion:The density gradient centrifugation technique combined withthe swim-up method is a stable and feasible method in D-IVF.关键词
供精/体外受精-胚胎移植/梯度密度离心法联合上游法/冷冻精液Key words
Donor/In vitro fertilization and embryo transfer/Density gradient centrifugation technique combined with the swim-up method/Frozen semen引用本文复制引用
谭玉梅,宋革,祝晓丽,郑炜炜..密度梯度离心联合上游法的精液处理在供精体外受精胚胎移植中的应用[J].中国医学创新,2015,(22):56-57,58,3.基金项目
广东省人口和计划生育委员会科研项目 （）
