中国药业Issue(15)：37-39,40,4.
高效液相色谱法测定愈伤灵胶囊中黄曲霉毒素 G2，G1，B2，B1含量
Determination of Aflatoxin G2, G1, B2, and B1 in Yushangling Capsules by HPLC
摘要
Abstract
Objective To establish a method for determination of aflatoxin G2, G1, B2, B1 in Yushangling capsules by HPLC. Methods The samples were extracted with 70% methanol and purified with immunoaffinity column, then analyzed by HPLC with fluorescence de-tection. The positive samples were identified by ultra performance liquid chromatography - tandem mass spectrometry. Results Aflatoxin G2, G1, B2, B1 showed a good linear relationship at a range of 3. 54 - 35. 40 pg, 7. 08 - 70. 80 pg, 2. 10 - 21. 00 pg, 6. 24 - 62. 40 pg ( r > 0. 999) . The recoveries were between 70% - 120% . Conclusion The method is simple, sensitive and accurate, which is suitable for determination of four aflatoxins in Yushangling Capsules.关键词
黄曲霉毒素/愈伤灵胶囊/免疫亲和柱/光化学衍生/高效液相色谱法/超高效液相色谱-串联质谱法Key words
aflatoxins/Yu Shangling capsules/immunoaffinity column/photochemical derivative/HPLC/UPLC - MS / MS分类
医药卫生引用本文复制引用
张正锋,张毅..高效液相色谱法测定愈伤灵胶囊中黄曲霉毒素 G2，G1，B2，B1含量[J].中国药业,2015,(15):37-39,40,4.基金项目
国家科技部重大新药创制专项，项目编号2014ZX09304－307－02。 （）
