中国卒中杂志Issue(8)：650-655,6.DOI:10.3969/j.issn.1673-5765.2015.08.004
15-LOX/15-HETE对Kv通道的抑制在缺氧诱导的脑动脉收缩中的作用
Inhibition of 15-LOX/15-HETE on the Expression of Kv Channels Participates in the Cerebral Vasoconstriction Induced by Hypoxia
摘要
Abstract
Objective To investigate the effect of the inhibition of 15-lipoxygenase (15-LOX)/15-hydroxyeicosatetraenoic acid (15-HETE) on the expression of Kv channels in the cerebral vasoconstriction induced by hypoxia. <br> Methods Smooth muscle cells originated from the cerebral artery of Wistar rats were separated and cultured by enzyme digestion method. The cells were then assigned randomly into four groups: group A:control group, carotid artery smooth muscle cells (CASMCs) were cultured under normoxic condition for 48h; group B:hypoxia group, CASMCs were cultured under hypoxic condition for 48h; group C:15-LOX gene overexpression under normoxic condition group, 15-LOX gene of CASMC was overexpressed and then the CASMCs were cultured under normoxic condition for 48h; and group D:15-LOX gene knockout under hypoxic condition group, 15-LOX gene of CASMC was knocked out and then the CASMCs were cultured under hypoxic condition for 48 h. The production of 15-HETE in each group was detected by using enzyme-linked immunosorbent assay (ELISA), the expression of Kv2.1 channel messenger ribonucleic acid (mRNA) and protein were detected by using reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis respectively. <br> Results After interference of 15-LOX gene on the CASMC, the production of 15-HETE and the expression of Kv2.1 were signiifcantly different from the normal cells group. The production of 15-HETE was higher and the expression of Kv2.1 channel mRNA and protein were lower in both hypoxia group and 15-LOX gene overexpression under normoxic condition group than control group; the production of 15-HETE was lower and the expression of Kv2.1 channel mRNA and protein were higher in 15-LOX gene knockout under hypoxic condition group than hypoxia group. <br> Conclusion The expression of 15-LOX increased in hypoxia, which increased the production of 15-HETE, and then leading to cerebral vasoconstriction via enhancing Kv2.1 channel inhibition. So the inhibition of 15-LOX/15-HETE on the Kv channels expression plays an important role that leads to cerebral vasoconstriction by hypoxia.关键词
15-脂加氧酶/15-羟二十碳四烯酸/Kv2.1/基因敲除/基因过表达Key words
15-lipoxygenase/15-hydroxyeicosatetraenoic acid/Kv2.1/Gene knockout/Gene overexpression引用本文复制引用
朱雨岚,王迪,刘文娟..15-LOX/15-HETE对Kv通道的抑制在缺氧诱导的脑动脉收缩中的作用[J].中国卒中杂志,2015,(8):650-655,6.基金项目
黑龙江省教育厅一般项目 （）
